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In this study, we investigated microbial communities occurring in the floral nectar of seven<jats:italic>Epipactis<\/jats:italic>(Orchidaceae) species. Culturable bacteria and yeasts were isolated and identified by partially sequencing the small subunit (SSU) ribosomal RNA (rRNA) gene and the D1\/D2 domains of the large subunit (LSU) rRNA gene, respectively. Using three different culture media, we found that bacteria were common inhabitants of the floral nectar of<jats:italic>Epipactis<\/jats:italic>. The most widely distributed bacterial operational taxonomic units (OTUs) in nectar of<jats:italic>Epipactis<\/jats:italic>were representatives of the family of Enterobacteriaceae<jats:italic>,<\/jats:italic>with an unspecified Enterobacteriaceae bacterium as the most common. In contrast to previous studies investigating microbial communities in floral nectar, very few yeast species (mainly of the genus<jats:italic>Cryptococcus<\/jats:italic>) were observed, and most of them occurred in very low densities. Total OTU richness (i.e., the number of bacterial and yeast OTUs per orchid species) varied between 4 and 20. Cluster analysis revealed that microbial communities of allogamous species differed from those of autogamous and facultatively autogamous species. This study extends previous efforts to identify microbial communities in floral nectar and indicates that the floral nectar of the orchids investigated mainly contained bacterial communities with moderate phylogenetic diversity.<\/jats:p>","DOI":"10.1002\/mbo3.103","type":"journal-article","created":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T09:08:38Z","timestamp":1373274518000},"page":"644-658","source":"Crossref","is-referenced-by-count":51,"title":["Microbial diversity in the floral nectar of seven<i>Epipactis<\/i>(Orchidaceae) species"],"prefix":"10.1002","volume":"2","author":[{"given":"Hans","family":"Jacquemyn","sequence":"first","affiliation":[{"name":"Plant Conservation and Population Biology Biology Department KU Leuven Kasteelpark Arenberg 31 B\u20103001 Heverlee Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijke","family":"Lenaerts","sequence":"additional","affiliation":[{"name":"Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&amp;BIM) Thomas More University College, De Nayer Campus Department of Microbial and Molecular Systems (M<sup>2<\/sup>S) KU Leuven Association B\u20102860 Sint\u2010Katelijne\u2010Waver Belgium"},{"name":"Scientia Terrae Research Institute B\u20102860 Sint\u2010Katelijne\u2010Waver Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Tyteca","sequence":"additional","affiliation":[{"name":"Biodiversity Research Centre Earth and Life Institute Universit\u00e9 catholique de Louvain B\u20101348 Louvain\u2010la\u2010Neuve Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Lievens","sequence":"additional","affiliation":[{"name":"Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&amp;BIM) Thomas More University College, De Nayer Campus Department of Microbial and Molecular Systems (M<sup>2<\/sup>S) KU Leuven Association B\u20102860 Sint\u2010Katelijne\u2010Waver Belgium"},{"name":"Scientia Terrae Research Institute B\u20102860 Sint\u2010Katelijne\u2010Waver Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780195182743.001.0001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2012.01329.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.043489-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000454107"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-4-1563"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-32892-0_6"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-011-9975-8"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2004.06.003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00937204"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcs183"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2004.05.008"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1461-0248.2004.00707.x"},{"key":"e_1_2_7_16_1","volume-title":"An atlas of orchid pollination, European orchids","author":"Cingel N. 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