{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:54:38Z","timestamp":1791320078285,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T00:00:00Z","timestamp":1584489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Centers for Disease Control and Prevention's Combating Antibiotic Resistance BAA","award":["200-2016-91939"],"award-info":[{"award-number":["200-2016-91939"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Community\u2010acquired urinary tract infections (UTIs) are one of the most common bacterial infections worldwide. <jats:italic>Escherichia coli<\/jats:italic> is the most common cause of community\u2010acquired UTI. In general, UTI results from <jats:italic>E.\u00a0coli<\/jats:italic> in the intestine that enters the bladder via the urethra. However, whether these <jats:italic>E.\u00a0coli<\/jats:italic> strains that cause UTI represent members of the intestinal commensal <jats:italic>E.\u00a0coli<\/jats:italic> or a distinct subgroup of pathogenic <jats:italic>E.\u00a0coli<\/jats:italic> remains unestablished. Here, we analyzed <jats:italic>E.\u00a0coli<\/jats:italic> isolates from fecal samples of healthy volunteers and urine samples of UTI patients obtained from a university\u2010affiliated health center. The <jats:italic>E.\u00a0coli<\/jats:italic> isolates were genotyped by multilocus sequence typing (MLST). From May to October 2018, we analyzed 89 <jats:italic>E.\u00a0coli<\/jats:italic> isolates from 76 (75%) rectal swabs from 113 unique healthy volunteers. We also analyzed 106 (27%) <jats:italic>E.\u00a0coli<\/jats:italic> isolates from 398 unique urine samples collected between August and October 2018. Fecal and urine <jats:italic>E.\u00a0coli<\/jats:italic> isolates each contained 31 distinct sequence types (STs). Nine STs were shared by fecal and urine <jats:italic>E.\u00a0coli<\/jats:italic> isolates, which accounted for approximately 50% of urine isolates typed by MLST. Among the shared genotypes, ST10 and ST131 were significantly more frequently found in fecal samples, whereas ST95 and ST127 were significantly more frequently recovered from UTI samples. ST73 was found only among urine samples. These <jats:italic>E.\u00a0coli<\/jats:italic> genotypes clustered and fluctuated over time. These observations suggest that <jats:italic>E.\u00a0coli<\/jats:italic> genotypes found to cause UTI transiently colonize the intestine and that their primary reservoir may reside outside of the human intestine.<\/jats:p>","DOI":"10.1002\/mbo3.1032","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T03:18:35Z","timestamp":1584587915000},"page":"1225-1233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Multilocus sequence typing of <i>Escherichia coli<\/i> isolates from urinary tract infection patients and from fecal samples of healthy subjects in a college community"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6016-2867","authenticated-orcid":false,"given":"Yusuke","family":"Matsui","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology School of Public Health University of California Berkeley CA 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