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In this study, the antifungal susceptibility test and multilocus sequence typing (MLST) analysis were carried out on <jats:italic>C<\/jats:italic>.<jats:italic>\u00a0tropicalis<\/jats:italic> from central China. Among 64 urogenital isolates, 45 diploid sequence types (DST) were found, of which 20 DSTs (44.4%) were new to the central database. The goeBURST analysis showed that CC1 (clonal complex) was the only azole\u2010resistant (100%, 10\/10) cluster in Wuhan, which was composed of DST546, DST225, DST376, and DST506, and most of the strains (90%, 9\/10) were isolated from the urinary tract. Potential nosocomial infections were mainly caused by CC1 strains. The azole resistance rate of urinary isolates (50.0%, 21\/42) was higher than that of vaginal isolates (27.3%, 6\/22). The genotype diversity and novelty of vaginal isolates were higher than those of urinary isolates. <jats:italic>C<\/jats:italic>.<jats:italic>\u00a0tropicalis<\/jats:italic> population in Wuhan was genetically diverse and divergent from that seen in other countries. In this study, there were significant differences in genotype and azole susceptibility between urine and vaginal strains. The azole\u2010resistant cluster (CC1) found in urine is of great significance for the clinical treatment and prevention of nosocomial infection. The newly discovered DSTs will contribute to further study the similarity, genetic relationship, and molecular epidemiology of <jats:italic>C<\/jats:italic>.<jats:italic>\u00a0tropicalis<\/jats:italic> worldwide.<\/jats:p>","DOI":"10.1002\/mbo3.1121","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T03:38:45Z","timestamp":1601264325000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular epidemiology of <i>Candida tropicalis<\/i> isolated from urogenital tract infections"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0486-3789","authenticated-orcid":false,"given":"Qianyu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congrong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongling","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewen","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,27]]},"reference":[{"issue":"8","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"e0182292","DOI":"10.1371\/journal.pone.0182292","article-title":"Population structure and molecular genetic characterization of clinical Candida tropicalis isolates from a tertiary\u2010care hospital in Kuwait reveal infections with unique strains","volume":"12","author":"Al\u2010Obaid K.","year":"2020","journal-title":"PLoS One"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765201520130418"},{"issue":"3","key":"e_1_2_10_4_1","first-page":"303","article-title":"Candida albicans in a neonatal intensive care unit: Antifungal susceptibility and genotypic analysis","volume":"30","author":"Asticcioli S.","year":"2007","journal-title":"New Microbiologica"},{"issue":"1","key":"e_1_2_10_5_1","first-page":"96","article-title":"Urinary tract infections and Candida albicans","volume":"68","author":"Behzadi P.","year":"2015","journal-title":"Central European Journal of Urology"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-007-0865-y"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/657320"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2509.190520"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.myc.2016.11.001"},{"issue":"6","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"3653","DOI":"10.1128\/AAC.02652-15","article-title":"Resistance mechanisms and clinical features of fluconazole\u2010nonsusceptible Candida tropicalis isolates compared with fluconazole\u2010less-susceptible isolates","volume":"60","author":"Choi M. 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