{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T07:12:35Z","timestamp":1778829155762,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T00:00:00Z","timestamp":1606348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DI 314\/12\u20102"],"award-info":[{"award-number":["DI 314\/12\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Energy conservation via organohalide respiration (OHR) in dehalogenating <jats:italic>Sulfurospirillum<\/jats:italic> species is an inducible process. However, the gene products involved in tetrachloroethene (PCE) sensing and signal transduction have not been unambiguously identified. Here, genome sequencing of <jats:italic>Sulfurospirillum<\/jats:italic> strains defective in PCE respiration and comparative genomics, which included the PCE\u2010respiring representatives of the genus, uncovered the genetic inactivation of a two\u2010component system (TCS) in the OHR gene region of the natural mutants. The assumption that the TCS gene products serve as a PCE sensor that initiates gene transcription was supported by the constitutive low\u2010level expression of the TCS operon in fumarate\u2010adapted cells of <jats:italic>Sulfurospirillum multivorans<\/jats:italic>. Via RNA sequencing, eight transcriptional units were identified in the OHR gene region, which includes the TCS operon, the PCE reductive dehalogenase operon, the gene cluster for norcobamide biosynthesis, and putative accessory genes with unknown functions. The OmpR\u2010family response regulator (RR) encoded in the TCS operon was functionally characterized by promoter\u2010binding assays. The RR bound a <jats:italic>cis<\/jats:italic>\u2010regulatory element that contained a consensus sequence of a direct repeat (CTATW) separated by 17\u00a0bp. Its location either overlapping the \u221235 box or 50\u00a0bp further upstream indicated different regulatory mechanisms. Sequence variations in the regulator binding sites identified in the OHR gene region were in accordance with differences in the transcript levels of the respective gene clusters forming the PCE regulon. The results indicate the presence of a fine\u2010tuned regulatory network controlling PCE metabolism in dehalogenating <jats:italic>Sulfurospirillum<\/jats:italic> species, a group of metabolically versatile organohalide\u2010respiring bacteria.<\/jats:p>","DOI":"10.1002\/mbo3.1138","type":"journal-article","created":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T15:13:19Z","timestamp":1606403599000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Tetrachloroethene respiration in <i>Sulfurospirillum<\/i> species is regulated by a two\u2010component system as unraveled by comparative genomics, transcriptomics, and regulator binding studies"],"prefix":"10.1002","volume":"9","author":[{"given":"Jens","family":"Esken","sequence":"first","affiliation":[{"name":"Department of Applied and Ecological Microbiology Institute of Microbiology Friedrich Schiller University Jena Germany"},{"name":"Leibniz Institute for Natural Product Research and Infection Biology \u2010 Hans Kn\u00f6ll Institute Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9977-5994","authenticated-orcid":false,"given":"Tobias","family":"Goris","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology Institute of Microbiology Friedrich Schiller University Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Gadkari","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology Institute of Microbiology Friedrich Schiller University Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9288-9481","authenticated-orcid":false,"given":"Thorsten","family":"Bischler","sequence":"additional","affiliation":[{"name":"Core Unit Systems Medicine University of W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konrad U.","family":"F\u00f6rstner","sequence":"additional","affiliation":[{"name":"ZB MED \u2010 Information Center for Life Sciences Cologne Germany"},{"name":"TH K\u00f6ln \u2010 University of Applied Sciences Institute of Information Science Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia M.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Core Unit Systems Medicine University of W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Diekert","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology Institute of Microbiology Friedrich Schiller University Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6569-1194","authenticated-orcid":false,"given":"Torsten","family":"Schubert","sequence":"additional","affiliation":[{"name":"Department of Applied and Ecological Microbiology Institute of Microbiology Friedrich Schiller University Jena Germany"},{"name":"Research Group Anaerobic Microbiology Institute of Microbiology Friedrich Schiller University Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1193"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt290"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1914"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258118"},{"issue":"5","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiy018","article-title":"Coexistence of two distinct Sulfurospirillum populations respiring tetrachloroethene \u2013 Genomic and kinetic considerations","volume":"94","author":"Buttet G. 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M.(2003).Characterization of tetrachloroethene dechlorinating cultures and isolation of a novel tetrachloroethene tocis\u20101 2\u2010dichloroethene halorespiring bacterium. PhD thesis at the Department of Civil and Environmental Engineering University of Washington."},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80136-7"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02173-12"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00262203"},{"issue":"4","key":"e_1_2_10_43_1","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiy035","article-title":"Organohalide respiratory chains: composition, topology and key enzymes","volume":"94","author":"Schubert T.","year":"2018","journal-title":"FEMS Microbiology Ecology"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08756"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2014.06.010"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900573"},{"issue":"2","key":"e_1_2_10_48_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0092-8674(80)90613-3","article-title":"E. coli RNA polymerase interacts homologously with two different promoters","volume":"20","author":"Siebenlist U.","year":"1980","journal-title":"Cell"},{"issue":"6","key":"e_1_2_10_49_1","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s00203-002-0473-8","article-title":"A non\u2010dechlorinating strain of Dehalospirillum multivorans: evidence for a key role of the corrinoid cofactor in the synthesis of an active tetrachloroethene dehalogenase","volume":"178","author":"Siebert A.","year":"2002","journal-title":"Archives of Microbiology"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.183"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv485"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.03.030"},{"issue":"3","key":"e_1_2_10_53_1","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiy013","article-title":"An integrative overview of genomic, transcriptomic and proteomic analyses in organohalide respiration research","volume":"94","author":"T\u00fcrkowsky D.","year":"2018","journal-title":"FEMS Microbiology Ecology"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0317"},{"issue":"682","key":"e_1_2_10_55_1","article-title":"Comparative genomic analysis of the class Epsilonproteobacteria and proposed reclassification to epsilonbacteraeota (phyl. nov.)","volume":"8","author":"Waite D. 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