{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T03:19:22Z","timestamp":1787800762018,"version":"build-2784847793"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,20]],"date-time":"2020-12-20T00:00:00Z","timestamp":1608422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072350"],"award-info":[{"award-number":["82072350"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The bacterium\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    has increasingly attracted global attention as an important opportunistic pathogen due to its ability to form biofilms that are known to increase drug resistance. However, there are still no effective antibiofilm drugs in clinical settings. Here, by drug repurposing, we investigated the antibacterial activity of penfluridol (PF), an oral long\u2010acting antipsychotic approved by the FDA, against\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0faecalis<\/jats:italic>\n                    type strain and its clinical isolates. It was found that PF inhibited the growth of\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0faecalis<\/jats:italic>\n                    planktonic cells with the MIC and MBC of 7.81\u00a0\u00b5g\/ml and 15.63\u00a0~\u00a062.50\u00a0\u00b5g\/ml, respectively. Moreover, PF could significantly prevent the biofilm formation of\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0faecalis<\/jats:italic>\n                    at the concentration of 1\u00a0\u00d7\u00a0MIC. Furthermore, PF significantly eradicated 24\u00a0h pre\u2010formed biofilms of\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0faecalis<\/jats:italic>\n                    in a dose\u2010dependent manner, with a concentration range of 1\u00a0\u00d7\u00a0MIC to 8\u00a0\u00d7\u00a0MIC. Here, through the checkerboard method with other tested conventional antibiotics, we also determined that gentamycin, penicillin G, and amikacin showed partial synergistic antibacterial effects with PF. Also, PF showed almost no hemolysis on human erythrocytes. In a mouse peritonitis model, a single dose of 20\u00a0mg\/kg of PF treatment could significantly reduce the bacterial colonization in the liver (~5\u2010fold reduction) and spleen (~3\u2010fold reduction). In conclusion, these findings indicated that after structural optimization, PF has the potential as a new antibacterial agent against\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0faecalis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/mbo3.1148","type":"journal-article","created":{"date-parts":[[2020,12,20]],"date-time":"2020-12-20T21:46:25Z","timestamp":1608500785000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Drug repurposing: Antimicrobial and antibiofilm effects of penfluridol against\n                    <i>Enterococcus faecalis<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Xianghai","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"She","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubair","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3667-8716","authenticated-orcid":false,"given":"Yong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192694"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2012.03.016"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm237"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2018.10.036"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.11.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2006.11.004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2015.01.002"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-009-9408-9"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.13439"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2012.00971.x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01957739"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01180-12"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.6.1173"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47331-0"},{"key":"e_1_2_10_17_1","first-page":"217","article-title":"Vancomycin\u2010resistant enterococcal infections: epidemiology, clinical manifestations, and optimal management","volume":"8","author":"O'Driscoll T.","year":"2015","journal-title":"Infection and Drug Resistance"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317705517"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1233"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-019-09618-6"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02453"},{"issue":"2","key":"e_1_2_10_22_1","first-page":"CD002923","article-title":"Penfluridol for schizophrenia","volume":"19","author":"Soares B. G.","year":"2006","journal-title":"The Cochrane Database of Systematic Reviews"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21030755"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126479"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02301"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MB-0001-2014"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24203659"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.4.5789.710"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-016-1081-1"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1148","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.1148","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1148","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T23:58:57Z","timestamp":1693353537000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.1148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,20]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/mbo3.1148"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.1148","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,20]]},"assertion":[{"value":"2020-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1148"}}