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In this study, we sequenced the full\u2010length of 16S rRNA and analyzed the differences in the intestinal microbiota between deltamethrin\u2010resistant and \u2010sensitive <jats:italic>Ae.\u00a0albopictus<\/jats:italic>. Symbiotic bacteria were cultured and analyzed using six types of culture media in aerobic and anaerobic environments. We found significant differences in the diversity and abundance of the intestinal microbiota of the two strains of <jats:italic>Ae.\u00a0albopictus<\/jats:italic>. The symbiotic bacteria cultured in vitro were found to be mainly facultative anaerobes. The cultured bacteria such as <jats:italic>Serratia oryzae<\/jats:italic> and <jats:italic>Acinetobacter junii<\/jats:italic> may function to promote the development of insecticide resistance. This work indicates that intestinal bacteria may contribute to the enhancement of insecticide resistance of <jats:italic>Ae. albopictus<\/jats:italic> It also highlights the analytical advantage of full\u2010length 16S rRNA sequencing to study the intestinal microbiota of mosquitoes.<\/jats:p>","DOI":"10.1002\/mbo3.1177","type":"journal-article","created":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T08:09:02Z","timestamp":1619856542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Differences in the intestinal microbiota between insecticide\u2010resistant and \u2010sensitive <i>Aedes albopictus<\/i> based on full\u2010length 16S rRNA sequencing"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3951-5901","authenticated-orcid":false,"given":"Haiyang","family":"Wang","sequence":"first","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongxing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maoqing","family":"Gong","sequence":"additional","affiliation":[{"name":"Shandong Institute of Parasitic Diseases Shandong First Medical University &amp; Shandong Academy of Medical Sciences Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006822"},{"key":"e_1_2_11_3_1","first-page":"762","article-title":"Serratia odorifera mediated enhancement in susceptibility of Aedes aegypti for chikungunya virus","volume":"139","author":"Apte\u2010Deshpande A. 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