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A new FDA\u2010cleared and CE\u2010marked platform utilizing magnetic resonance to detect amplified DNA of the six most common and\/or problematic BSI pathogens (<jats:italic>Enterococcus faecium<\/jats:italic>, <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Acinetobacter baumannii<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, and <jats:italic>Escherichia coli<\/jats:italic>; referred to as ESKAPEc) is available and may shorten the time to diagnosis and potentially improve antimicrobial utilization. Whole blood samples from hospitalized patients with clinical signs of sepsis were analyzed using the T2Bacteria Panel (T2Biosystems) and compared to simultaneously collected BC. Discrepant results were evaluated based on clinical infection criteria, combining supporting culture results and the opinion of treating physicians. A total of 55\u00a0samples from 53 patients were evaluated. The sensitivity and specificity of the T2Bacteria panel was 94% (16 out of 17 detections of T2Bacteria\u2010targeted organisms) and 100%, respectively, with 36.4% (8 of 22) causes of BSI detected only by this method. The T2Bacteria Panel detected pathogens on average 55\u00a0hours faster than standard BC. In our study, 9 of 15 patients with positive T2Bacteria Panel results received early\u2010targeted antibiotic therapy and\/or modification of antimicrobial treatment based on T2Bacteria Panel findings. Given the high reliability, faster time to detection, and easy workflow, the technique qualifies as a point\u2010of\u2010care testing approach.<\/jats:p>","DOI":"10.1002\/mbo3.1210","type":"journal-article","created":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T04:04:00Z","timestamp":1623902640000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Direct detection of ESKAPEc pathogens from whole blood using the T2Bacteria Panel allows early antimicrobial stewardship intervention in patients with sepsis"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8994-5240","authenticated-orcid":false,"given":"Pavel","family":"Drevinek","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology 2nd Faculty of Medicine Charles University Motol University Hospital Prague Czech 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