{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:06:15Z","timestamp":1787508375979,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T00:00:00Z","timestamp":1633651200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptococcus suis<\/jats:italic>\n                    , an emerging zoonotic pathogen, causes invasive diseases in pigs, including sepsis, meningitis, endocarditis, pneumonia, and arthritis. Importantly, similar pathologies are reported in human\n                    <jats:italic>S<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>suis<\/jats:italic>\n                    infections. In previous work, the locus SSU0375 of\n                    <jats:italic>S<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>suis<\/jats:italic>\n                    strain P1.7 had been identified as a conditionally essential gene by intrathecal experimental infection of pigs with a transposon library of\n                    <jats:italic>S<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>suis<\/jats:italic>\n                    . This study aimed to identify the function of the corresponding gene product. Bioinformatics analysis and homology modeling revealed sequence and structural homologies with the\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    mid\u2010cell\u2010anchored protein Z (MapZ) that is involved in cell division in different bacterial species. Indeed, depletion of this locus in\n                    <jats:italic>S<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>suis<\/jats:italic>\n                    strain 10 revealed a growth defect as compared to the wild type. Electron microscopy analysis of the corresponding mutant demonstrated morphological growth defects as compared to the wild\u2010type strain, including an irregular cell shape and size as well as mispositioned division septa. Light microscopy and subsequent quantitative image analysis confirmed these morphological alterations. In the genetic rescue strain, the wild\u2010type phenotype was completely restored. In summary, we proposed that SSU0375 or the corresponding locus in strain 10 encode for a\n                    <jats:italic>S<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>suis<\/jats:italic>\n                    MapZ homolog that guides septum positioning as evidenced for other members of the Streptococci family.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.1234","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T09:12:51Z","timestamp":1633857171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification and characterization of the cell division protein MapZ from\n                    <i>Streptococcus suis<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Muriel","family":"Dresen","sequence":"first","affiliation":[{"name":"Institute for Microbiology Center for Infection Medicine University of Veterinary Medicine Hannover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Rohde","sequence":"additional","affiliation":[{"name":"Central Facility for Microscopy Helmholtz Centre for Infection Research  Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8134-0693","authenticated-orcid":false,"given":"Jes\u00fas","family":"Arenas","sequence":"additional","affiliation":[{"name":"Unit of Microbiology and Immunology Faculty of Veterinary University of Zaragoza  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2498-3668","authenticated-orcid":false,"given":"Astrid","family":"de Greeff","sequence":"additional","affiliation":[{"name":"Wageningen Bioveterinary Research Part of Wageningen University and Research  Lelystad The 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