{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T20:01:06Z","timestamp":1776715266998,"version":"3.51.2"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770086"],"award-info":[{"award-number":["31770086"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31861133014"],"award-info":[{"award-number":["31861133014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900404"],"award-info":[{"award-number":["31900404"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In fungal pathogens, the transcription factor SrbA (a <jats:styled-content>s<\/jats:styled-content>terol <jats:styled-content>r<\/jats:styled-content>egulatory element\u2010<jats:styled-content>b<\/jats:styled-content>inding protein, SREBP) and CBC (<jats:styled-content>C<\/jats:styled-content>CAAT <jats:styled-content>b<\/jats:styled-content>inding <jats:styled-content>c<\/jats:styled-content>omplex) have been reported to regulate azole resistance by competitively binding the TR34 region (34 <jats:italic>mer<\/jats:italic>) in the promoter of the drug target gene, <jats:italic>erg11A<\/jats:italic>. However, current knowledge about how the SrbA and CBC coordinately mediate <jats:italic>erg11A<\/jats:italic> expression remains limited. In this study, we uncovered a novel relationship between HapB (a subunit of CBC) and SrbA in which deletion of <jats:italic>hapB<\/jats:italic> significantly prolongs the nuclear retention of SrbA by increasing its expression and cleavage under azole treatment conditions, thereby enhancing Erg11A expression for drug resistance. Furthermore, we verified that loss of HapB significantly induces the expression of the rhomboid protease RbdB, Dsc ubiquitin E3 ligase complex, and signal peptide peptidase SppA, which are required for the cleavage of SrbA, suggesting that HapB acts as a repressor for these genes which contribute to the activation of SrbA by proteolytic cleavage. Together, our study reveals that CBC functions not only to compete with SrbA for binding to <jats:italic>erg11A<\/jats:italic> promoter region but also to affect SrbA expression, cleavage, and translocation to nuclei for the function, which ultimately regulate Erg11A expression and azole resistance.<\/jats:p>","DOI":"10.1002\/mbo3.1249","type":"journal-article","created":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T01:55:36Z","timestamp":1637632536000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The CCAAT\u2010binding complex mediates azole susceptibility of <i>Aspergillus fumigatus<\/i> by suppressing SrbA expression and cleavage"],"prefix":"10.1002","volume":"10","author":[{"given":"Chi","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Gao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiran","family":"Ren","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiyu","family":"Gu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0854-6123","authenticated-orcid":false,"given":"Yuanwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2891-7326","authenticated-orcid":false,"given":"Ling","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1997.0980"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.1996.tb02629.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13341"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010012\u20104195\u20109"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002374"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05027\u201011"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)80213\u20105"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753\u20104887.1998.tb01680.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11041"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009711"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050034"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix210"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004487"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00041\u201008"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa6097"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00035\u201016"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01839\u201020"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aap7999"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa109"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201014191\u20101"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201005497\u20107"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.279"},{"issue":"9","key":"e_1_2_11_24_1","first-page":"661","article-title":"Pramiconazole, a triazole compound for the treatment of fungal infections","volume":"11","author":"Geria A. N.","year":"2008","journal-title":"Idrugs"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12751"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005775"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006096"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2016.11.008"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.12451"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.1.184\u2010190.1996"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007762"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01092\u201006"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr025541x"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02563\u201018"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03007\u201021"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00045\u201010"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050219"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010016\u20108072\u20109"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4412"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1159\/000331860"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/Iai.00011\u201016"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02101\u201016"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/Ec.00252\u201012"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000200"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20107104\u20106_17"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.118.200253"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.12.007"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00691"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1249","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.1249","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T05:51:07Z","timestamp":1693115467000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.1249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,22]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/mbo3.1249"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.1249","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,22]]},"assertion":[{"value":"2021-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1249"}}