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Here, RNA\u2010seq was used to perform transcriptomic analysis of <jats:italic>S. agalactiae<\/jats:italic> PJI using fluid derived from sonication of explanted arthroplasties of subjects with <jats:italic>S. agalactiae<\/jats:italic> PJI, with results compared to those of <jats:italic>S. agalactiae<\/jats:italic> strain NEM316 grown in vitro. A total of 227 genes with outlier expression were found (164 upregulated and 63 downregulated) between PJI sonicate fluid and in vitro conditions. Functional enrichment analysis showed genes involved in mobilome and inorganic ion transport and metabolism to be most enriched. Genes involved in nickel, copper, and zinc transport, were upregulated. Among known virulence factors, <jats:italic>cyl<\/jats:italic> operon genes, encoding \u03b2\u2010hemolysin\/cytolysin, were consistently highly expressed in PJI <jats:italic>versus<\/jats:italic> in vitro. The data presented provide insight into <jats:italic>S. agalactiae<\/jats:italic> PJI pathogenesis and may be a resource for identification of novel PJI therapeutics or vaccines against invasive <jats:italic>S. agalactiae<\/jats:italic> infections.<\/jats:p>","DOI":"10.1002\/mbo3.1256","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T09:30:17Z","timestamp":1639733417000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transcriptomic analysis of <i>Streptococcus agalactiae<\/i> periprosthetic joint infection"],"prefix":"10.1002","volume":"10","author":[{"given":"Hye\u2010Kyung","family":"Cho","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thao","family":"Masters","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerryl E.","family":"Greenwood\u2010Quaintance","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Research Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio R.","family":"Jeraldo","sequence":"additional","affiliation":[{"name":"Center for Individualized Medicine Mayo Clinic Rochester Minnesota USA"},{"name":"Department of Surgery Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Chia","sequence":"additional","affiliation":[{"name":"Center for Individualized Medicine Mayo Clinic Rochester Minnesota USA"},{"name":"Department of Surgery Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Pu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology and Hepatology Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew P.","family":"Abdel","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-4141","authenticated-orcid":false,"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology Mayo Clinic Rochester Minnesota USA"},{"name":"Division of Infectious Diseases, Department of Medicine Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.99B5.BJJ-2016-0851.R1"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt389"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00325"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07862.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-009-0945-0"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.H.00155"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3519"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2018.02.062"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu558"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3096-3103.2005"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00762-17"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152449"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00581-10"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.09.009"},{"key":"e_1_2_11_16_1","article-title":"The PATRIC Bioinformatics Resource Center: Expanding data and analysis capabilities","volume":"35","author":"Davis J. 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