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This study aimed to investigate the sporicidal effects of sertaconazole nitrate using microplate laser nephelometry against the microconidia of <jats:italic>Trichophyton<\/jats:italic>, chlamydospores of <jats:italic>Epidermophyton<\/jats:italic>, blastospores of <jats:italic>Candida<\/jats:italic>, and conidia of the mold <jats:italic>Scopulariopsis brevicaulis<\/jats:italic>. The results obtained were compared with those from ciclopirox olamine and terbinafine. The sporicidal activity was further determined using infected three\u2010dimensional full skin models to determine the antifungal effects in the presence of human cells. Sertaconazole nitrate inhibited the growth of dermatophytes, molds, and yeasts. Ciclopirox olamine also had good antifungal activity, although higher concentrations were needed compared to sertaconazole nitrate. Terbinafine was highly effective against most dermatophytes, but higher concentrations were required to kill the resistant strain <jats:italic>Trichophyton indotineae<\/jats:italic>. Sertaconazole nitrate, ciclopirox olamine, and terbinafine had no negative effects on full skin models. Sertaconazole nitrate reduced the growth of fungal and yeast spores over 72\u2009h. Ciclopirox olamine and terbinafine also inhibited the growth of dermatophytes and molds but had significantly lower effects on the yeast. Sertaconazole nitrate might have advantages over the commonly used antifungals ciclopirox olamine and terbinafine in combating resting spores, which persist in the tissues, and thus in the therapy of recurring dermatomycoses.<\/jats:p>","DOI":"10.1002\/mbo3.1257","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T12:21:27Z","timestamp":1642076487000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Efficacy of antifungal agents against fungal spores: An in vitro study using microplate laser nephelometry and an artificially infected 3D skin model"],"prefix":"10.1002","volume":"11","author":[{"given":"Sarah","family":"Fink","sequence":"first","affiliation":[{"name":"Department of Dermatology University Hospital Jena Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anke","family":"Burmester","sequence":"additional","affiliation":[{"name":"Department of 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