{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:24:28Z","timestamp":1782894268087,"version":"3.54.5"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["20\/FFP\u2010P\/8717"],"award-info":[{"award-number":["20\/FFP\u2010P\/8717"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Universal stress proteins (USPs) are ubiquitously expressed in bacteria, archaea, and eukaryotes and play a lead role in adaptation to environmental conditions. They enable adaptation of bacterial pathogens to the conditions encountered in the human niche, including hypoxia, oxidative stress, osmotic stress, nutrient deficiency, or acid stress, thereby facilitating colonization. We previously reported that all six USP proteins encoded within a low\u2010oxygen activated (\n                    <jats:italic>lxa<\/jats:italic>\n                    ) locus in\n                    <jats:italic>Burkholderia cenocepacia<\/jats:italic>\n                    showed increased abundance during chronic colonization of the cystic fibrosis\u00a0(CF) lung. However, the role of USPs in chronic cystic fibrosis infection is not well understood. Structural modeling identified surface arginines on one\n                    <jats:italic>lxa<\/jats:italic>\n                    \u2010encoded USP, USP76, which suggested it mediated interactions with heparan sulfate. Using mutants derived from the\n                    <jats:italic>B. cenocepacia<\/jats:italic>\n                    strain, K56\u20102, we show that USP76 is involved in host cell attachment. Pretreatment of lung epithelial cells with heparanase reduced the binding of the wild\u2010type and complement strains but not the \u0394\n                    <jats:italic>usp76<\/jats:italic>\n                    mutant strain, indicating that USP76 is directly or indirectly involved in receptor recognition on the surface of epithelial cells. We also show that USP76 is required for growth and survival in many conditions associated with the CF lung, including acidic conditions and oxidative stress. Moreover, USP76 also has a role in survival in macrophages isolated from people with CF. Overall, while further elucidation of the exact mechanism(s) is required, we can conclude that USP76, which is upregulated during chronic infection, is involved in bacterial survival within CF macrophages, a hallmark of\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.1311","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T09:16:05Z","timestamp":1670577365000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A universal stress protein upregulated by hypoxia has a role in\n                    <i>Burkholderia cenocepacia<\/i>\n                    intramacrophage survival: Implications for chronic infection in cystic fibrosis"],"prefix":"10.1002","volume":"12","author":[{"given":"Andrew","family":"O'Connor","sequence":"first","affiliation":[{"name":"School of Biomolecular and Biomedical 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