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The increase of brucellosis among several species of cetaceans and pinnipeds, together with the report of sporadic human infections, raises concerns about the zoonotic potential of these pathogens on a large scale and may pose a threat to coastal communities worldwide. Therefore, the characterization of the <jats:italic>B. ceti<\/jats:italic> and <jats:italic>B. pinnipedialis<\/jats:italic> genetic features is a priority to better understand the pathological factors that may impact global health. Moreover, an in\u2010depth functional analysis of the <jats:italic>B. ceti<\/jats:italic> and <jats:italic>B. pinnipedialis<\/jats:italic> genome in the context of virulence and pathogenesis was not undertaken so far. Within this picture, here we present the comparative whole\u2010genome characterization of all <jats:italic>B. ceti<\/jats:italic> and <jats:italic>B. pinnipedialis<\/jats:italic> genomes available in public resources, uncovering a collection of genetic tools possessed by these aquatic bacterial species compared to their zoonotic terrestrial relatives. We show that <jats:italic>B. ceti<\/jats:italic> and <jats:italic>B. pinnipedialis<\/jats:italic> genomes display a wide host\u2010range infection capability and a polyphyletic phylogeny within the genus, showing a genomic structure that fits the canonical definition of closeness. Functional genome annotation led to identifying genes related to several pathways involved in mechanisms of infection, others conferring pan\u2010susceptibility to antimicrobials and a set of virulence genes that highlight the similarity of <jats:italic>B. ceti<\/jats:italic> and <jats:italic>B. pinnipedialis<\/jats:italic> genotypes to those of <jats:italic>Brucella<\/jats:italic> spp. displaying human\u2010infecting phenotypes.<\/jats:p>","DOI":"10.1002\/mbo3.1329","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T07:21:20Z","timestamp":1666768880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<i>Brucella ceti<\/i> and <i>Brucella pinnipedialis<\/i> genome characterization unveils genetic features that highlight their zoonotic potential"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0087-3349","authenticated-orcid":false,"given":"Massimiliano","family":"Orsini","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Laboratory of Microbial Ecology and Genomics  Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ianni","sequence":"additional","affiliation":[{"name":"Research Unit in Hygiene, Statistics and Public Health Campus Bio\u2010Medico di Roma University Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6489-7070","authenticated-orcid":false,"given":"Luca","family":"Zinzula","sequence":"additional","affiliation":[{"name":"Department of Molecular Structural Biology Max Planck Institute of Biochemistry  Martinsried Germany"},{"name":"Centro di Educazione Ambientale e alla Sostenibilit\u00e0 (CEAS) Laguna di Nora  Pula Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz935"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1202"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw387"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102905"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-11-200"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01601.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.117672"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032514299"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02101"},{"key":"e_1_2_10_14_1","first-page":"483","article-title":"Human exposure to Brucella recovered from a sea mammal","volume":"144","author":"Brew S. 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