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We have isolated a strain from a purified diet intended for research animals that further demonstrates the ability of <jats:italic>E. coli<\/jats:italic> to acquire novel genetic elements leading potentially to emergent new pathovars. Utilizing next generation sequencing to obtain a whole genome profile, we report an atypical strain of <jats:italic>E. coli<\/jats:italic>, EcoFA807\u201017, possessing a tetrathionate reductase (<jats:italic>ttr<\/jats:italic>) operon, which enables the utilization of tetrathionate as an electron acceptor, thus facilitating respiration in anaerobic environments such as the mammalian gut. The <jats:italic>ttr<\/jats:italic> operon is a potent virulence factor for several enteric pathogens, most prominently <jats:italic>Salmonella enterica<\/jats:italic>. However, the presence of chromosomally integrated tetrathionate reductase genes does not appear to have been previously reported in wild\u2010type <jats:italic>E. coli<\/jats:italic> or <jats:italic>Shigella<\/jats:italic>. Accordingly, it is possible that the appearance of this virulence factor may signal the evolution of new mechanisms of pathogenicity in <jats:italic>E. coli<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> and may potentially alter the effectiveness of existing assays using tetrathionate reductase as a unique marker for the detection of <jats:italic>Salmonella enterica<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/mbo3.1333","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T07:18:51Z","timestamp":1667805531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The emergence of the tetrathionate reductase operon in the <i>Escherichia coli\/Shigella<\/i> pan\u2010genome"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1971-3604","authenticated-orcid":false,"suffix":"Jr","given":"Floyd 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