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Its pathogenicity is associated with the production of virulence factors such as extracellular hydrolytic enzymes and toxins. This study was aimed at determining the effect of external pH, substrate nature, and strain origin on protease, lipase, and hemolysin production. To achieve this objective, agar plate assays were performed at pH 5.0, 6.5, and 7.5 with substrates suitable for the detection of each family of enzymes. Moreover, the study was conducted with 20 clinical <jats:italic>C. albicans<\/jats:italic> isolates from blood, oral cavity, skin, urine, and vagina. The hydrolytic zones formed around the colonies were further measured to calculate the <jats:italic>Ez<\/jats:italic> (enzymatic zone) indexes. We found that detection of proteases in skim milk agar plates was possible for most isolates only at pH 5 (80%) and pH 6.5 (75%), whereas BSA plates could confer protease detection exclusively at pH 5 (80%). Similarly, the percentage of isolates possessing lipolytic activities was higher at pH 5 (90%) than at pH 6.5 (70%) and pH 7.5 (35%). In contrast, hemolytic activities were detected in all isolates at pH 6.5 and 7.5 but not at pH 5. Further analysis revealed that some differences in the detected activities could potentially be attributed to the anatomical origin of these isolates. Collectively, these findings suggest that the pH of the site of infection might be critical for mimicking the microenvironment employed to experimentally discover the key virulence factors.<\/jats:p>","DOI":"10.1002\/mbo3.1342","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T08:46:20Z","timestamp":1672735580000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Assessing pH\u2010dependent activities of virulence factors secreted by <i>Candida albicans<\/i>"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5272-5757","authenticated-orcid":false,"given":"Asier","family":"Ramos\u2010Pardo","sequence":"first","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology University of the Basque Country UPV\/EHU Leioa 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