{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:01:24Z","timestamp":1753869684749,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We present a comprehensive sequence and bioinformatic analysis of the prototypical microcin plasmid, pMccb17, which includes a definitive sequence for the microcin operon, <jats:italic>mcb<\/jats:italic>. Microcin B17 (MccB17) is a ribosomally synthesized and posttranslationally modified peptide produced by <jats:italic>Escherichia coli<\/jats:italic>. It inhibits bacterial DNA gyrase similarly to quinolone antibiotics. The <jats:italic>mcb<\/jats:italic> operon, which consists of seven genes encoding biosynthetic and immunity\/export functions, was originally located on the low copy number IncFII plasmid pMccB17 in the <jats:italic>Escherichia coli<\/jats:italic> strain LP17. It was later transferred to <jats:italic>E. coli<\/jats:italic> K\u201012 through conjugation. In this study, the plasmid was extracted from the <jats:italic>E. coli<\/jats:italic> K\u201012 strain RYC1000 [pMccB17] and sequenced twice using an Illumina short\u2010read method. The first sequencing was conducted with the host bacterial chromosome, and the plasmid DNA was then purified and sequenced separately. After assembly into a single contig, polymerase chain reaction primers were designed to close the single remaining gap via Sanger sequencing. The resulting complete circular DNA sequence is 69,190\u2009bp long and includes 81 predicted genes. These genes were initially identified by Prokka and subsequently manually reannotated using BLAST. The plasmid was assigned to the F2:A\u2010:B\u2010 replicon type with a MOBF12 group conjugation system. A comparison with other IncFII plasmids revealed a large proportion of shared genes, particularly in the conjugative plasmid backbone. However, unlike many contemporary IncFII plasmids, pMccB17 lacks transposable elements and antibiotic resistance genes. In addition to the <jats:italic>mcb<\/jats:italic> operon, this plasmid carries 25 genes of unknown function.<\/jats:p>","DOI":"10.1002\/mbo3.1402","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T10:18:46Z","timestamp":1709633926000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Complete nucleotide sequence and comparative genomic analysis of microcin B17 plasmid pMccB17"],"prefix":"10.1002","volume":"13","author":[{"given":"Mayokun","family":"Ajeigbe","sequence":"first","affiliation":[{"name":"School of Nursing and Health Sciences, Faculty of Health Sciences and Wellbeing University of Sunderland Sunderland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Childs","sequence":"additional","affiliation":[{"name":"School of Pharmacy &amp; Pharmaceutical Sciences, Faculty of Health Sciences and Wellbeing University of Sunderland Sunderland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy A.","family":"Paget","sequence":"additional","affiliation":[{"name":"School of Medicine, Faculty of Health Sciences and Wellbeing University of Sunderland Sunderland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5168-5167","authenticated-orcid":false,"given":"Lewis E. H.","family":"Bingle","sequence":"additional","affiliation":[{"name":"School of Nursing and Health Sciences, Faculty of Health Sciences and Wellbeing University of Sunderland Sunderland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2NP20085F"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.135.2.342-347.1978"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/4.2.124"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn529"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn578"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti553"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.05.050"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061459"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/b516237h"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2016.00071"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00291.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9877-7_21"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03018.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.2.1126-1135.1989"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.11.032"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4562"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msn067"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1080"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00261174"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1390"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90057-4"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2023.102289"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304516200"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.10.944"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.163.1.275-281.1985"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-3-r30"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq347"},{"key":"e_1_2_10_36_1","doi-asserted-by":"crossref","unstructured":"Withanage S. Masschelein J. &Pinheiro V. B.(2013).Escherichia coli Microcin B17 as a chassis for the development of novel antimicrobial peptides.https:\/\/doi.org\/10.1101\/2023.08.29.555279.","DOI":"10.1101\/2023.08.29.555279"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.10.4519"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/10665270050081478"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2009.02614.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T10:09:24Z","timestamp":1714039764000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.1402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,5]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/mbo3.1402"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.1402","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"type":"print","value":"2045-8827"},{"type":"electronic","value":"2045-8827"}],"subject":[],"published":{"date-parts":[[2024,3,5]]},"assertion":[{"value":"2023-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1402"}}