{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:53:56Z","timestamp":1790873636919,"version":"4.1.0"},"reference-count":96,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microbial communities from various environments have been studied in the quest for new natural products with a broad range of applications in medicine and biotechnology. We employed an enrichment method and genome mining tools to examine the biosynthetic potential of microbial communities in the sediments of a coastal sinkhole within the karst ecosystem of the Yucat\u00e1n Peninsula, Mexico. Our investigation led to the detection of 203 biosynthetic gene clusters (BGCs)\u00a0and 55 secondary metabolites (SMs)\u00a0within 35 high\u2010quality metagenome\u2010assembled genomes (MAGs)\u00a0derived from these subcommunities. The most abundant types of BGCs were Terpene, Nonribosomal peptide\u2010synthetase, and Type III polyketide synthase. Some of the in silico identified BGCs and SMs have been previously reported to exhibit biological activities against pathogenic bacteria and fungi. Others could play significant roles in the sinkhole ecosystem, such as iron solubilization and osmotic stress protection. Interestingly, 75% of the BGCs showed no sequence homology with bacterial BGCs previously reported in the MiBIG database. This suggests that the microbial communities in this environment could be an untapped source of genes encoding novel specialized compounds. The majority of the BGCs were identified in pathways found in the genus <jats:italic>Virgibacillus<\/jats:italic>, followed by <jats:italic>Sporosarcina, Siminovitchia, Rhodococcus<\/jats:italic>, and <jats:italic>Halomonas<\/jats:italic>. The latter, along with <jats:italic>Paraclostridium<\/jats:italic> and <jats:italic>Lysinibacillus<\/jats:italic>, had the highest number of identified BGC types. This study offers fresh insights into the potential ecological role of SMs from sediment microbial communities in an unexplored environment, underscoring their value as a source of novel natural products.<\/jats:p>","DOI":"10.1002\/mbo3.1407","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T19:11:28Z","timestamp":1712689888000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7918-3000","authenticated-orcid":false,"given":"Pablo","family":"Su\u00e1rez\u2010Moo","sequence":"first","affiliation":[{"name":"Unidad de Qu\u00edmica\u2010Sisal, Facultad de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico  Sisal Yucat\u00e1n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6122-9923","authenticated-orcid":false,"given":"Alejandra","family":"Prieto\u2010Dav\u00f3","sequence":"additional","affiliation":[{"name":"Unidad de Qu\u00edmica\u2010Sisal, Facultad de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico  Sisal Yucat\u00e1n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.margen.2020.100782"},{"key":"e_1_2_10_3_1","unstructured":"Andrews S.(2010).Data\u00a0FastQC: A quality control tool for high throughput sequence."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118235"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10040-010-0699-5"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab335"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz310"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3893"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01776-x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2008.08.009"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-13409-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3966-1"},{"key":"e_1_2_10_14_1","unstructured":"Chase A. B. Sweeney D. Muskat M. N. Guill\u00e9n\u2010matus D. &Jensen P. R.(2021).Evolution and chemical diversification evolution and chemical diversification."},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz848"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-35"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.258640.119"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines6010032"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01950"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.595991"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02510-6"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00251"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes9040177"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2010.02.010"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-611"},{"key":"e_1_2_10_26_1","first-page":"83","article-title":"The Genus Rhodococcus as a source of novel bioactive substances: A review","volume":"6","author":"Elsayed Y.","year":"2017","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690451"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fny202"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-98141-3"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004475"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-019-2707-9"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812082106"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b02513"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.01026-22"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7359"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b00110"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-015-1723-5"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jph.12500"},{"key":"e_1_2_10_40_1","first-page":"31","volume-title":"Peptide antibiotics produced\u00a0by\u00a0Bacillus species:\u00a0First line\u00a0of\u00a0attack\u00a0in the\u00a0biocontrol\u00a0of\u00a0plant diseases","author":"Khatoon Z.","year":"2022"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz188"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz239"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13867"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2np20103h"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1109\/TVCG.2014.2346248"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv033"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV119.006545"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-021-01567-6"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj-cs.104"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26237332"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-016-6092-x"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1890.Minimum"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0ob01677b"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2019-0173"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0718-6"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-11917-y"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-022-09952-2"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6065-7"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2010.06.005"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14471"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6635-9"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1099\/MGEN.0.000731"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386905-0.00002-4"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.5194\/bg-16-2285-2019"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22063253"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2584"},{"key":"e_1_2_10_70_1","first-page":"231","article-title":"Microbial diversity, metagenomics and the Yucat\u00e1n aquifer","volume":"80","author":"Rojas\u2010Herrera R.","year":"2011","journal-title":"Phyton (B Aires)"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2007.02185.x"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14386"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24133-5"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014923217206"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13398"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-47879-x"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.3390\/md14040072"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00057-21"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.palaeo.2017.10.012"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02997-19"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-05135-9"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.064360-0"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202003411"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5453.640"},{"key":"e_1_2_10_85_1","unstructured":"RStudio Team. (2015).RStudio: Integrated development for R."},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biombioe.2020.105677"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01166-y"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky383"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02781-21"},{"key":"e_1_2_10_90_1","unstructured":"Warnes G. R. Bolker B. Bonebakker L. Gentleman R. Liaw W. H. A. Lumley T. Maechler M. Magnusson A. Moeller S. Schwartz M. &Venables B.(2016).gplots: Vario\u00a0us R programming tools for plotting data. Version 3.0.1."},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2008.10.005"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-3-r46"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1422108112"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2022.134109"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2021.01.023"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1324161111"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034064"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T10:07:25Z","timestamp":1714039645000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.1407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":96,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/mbo3.1407"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.1407","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1407"}}