{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:58Z","timestamp":1786978198369,"version":"build-2736575974"},"reference-count":73,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and dechlorination rates and extent. We investigate the role of cobamide type on carbon and chlorine isotopic signals observed during reductive dechlorination of chloroform by the RDase CfrA. Microcosm experiments with two subcultures of a\n                    <jats:italic>Dehalobacter<\/jats:italic>\n                    \u2010containing culture expressing CfrA\u2014one with exogenous cobamide (Vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    , B12\n                    <jats:sup>+<\/jats:sup>\n                    ) and one without (to drive native cobamide production)\u2014resulted in a markedly smaller carbon isotope enrichment factor (\n                    <jats:italic>\u03b5<\/jats:italic>\n                    <jats:sub>C, bulk<\/jats:sub>\n                    ) for B12\n                    <jats:sup>\u2212<\/jats:sup>\n                    (\u221222.1\u2009\u00b1\u20091.9\u2030) compared to B12\n                    <jats:sup>+<\/jats:sup>\n                    (\u221226.8\u2009\u00b1\u20093.2\u2030). Both cultures exhibited significant chlorine isotope fractionation, and although a lower\n                    <jats:italic>\u03b5<\/jats:italic>\n                    <jats:sub>Cl, bulk<\/jats:sub>\n                    was observed for B12\n                    <jats:sup>\u2212<\/jats:sup>\n                    (\u22126.17\u2009\u00b1\u20090.72\u2030) compared to B12\n                    <jats:sup>+<\/jats:sup>\n                    (\u22126.86\u2009\u00b1\u20090.77\u2030) cultures, these values are not statistically different. Importantly, dual\u2010isotope plots produced identical slopes of\n                    <jats:italic>\u039b<\/jats:italic>\n                    <jats:sub>Cl\/C<\/jats:sub>\n                    (\n                    <jats:italic>\u039b<\/jats:italic>\n                    <jats:sub>Cl\/C, B12+<\/jats:sub>\n                    \u2009=\u20093.41\u2009\u00b1\u20090.15,\n                    <jats:italic>\u039b<\/jats:italic>\n                    <jats:sub>Cl\/C, B12<\/jats:sub>\n                    \u2212\u2009=\u20093.39\u2009\u00b1\u20090.15), suggesting the same reaction mechanism is involved in both experiments, independent of the lower cobamide bases. A nonisotopically fractionating masking effect may explain the smaller fractionations observed for the B12\n                    <jats:sup>\u2212<\/jats:sup>\n                    containing culture.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.1433","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T10:39:25Z","timestamp":1724755165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Vitamin B\n                    <sub>12<\/sub>\n                    as a source of variability in isotope effects for chloroform biotransformation by\n                    <i>Dehalobacter<\/i>"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3412-5480","authenticated-orcid":false,"given":"Elizabeth","family":"Phillips","sequence":"first","affiliation":[{"name":"Department of Earth Sciences University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Picott","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"K\u00fcmmel","sequence":"additional","affiliation":[{"name":"Department of Technical Biogeochemistry Helmholtz Centre for Environmental Research\u2014UFZ Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Bulka","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Hsiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Gehre","sequence":"additional","affiliation":[{"name":"Department of Technical Biogeochemistry Helmholtz Centre for Environmental Research\u2014UFZ Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivonne","family":"Nijenhuis","sequence":"additional","affiliation":[{"name":"Department of Technical Biogeochemistry Helmholtz Centre for Environmental Research\u2014UFZ Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara S.","family":"Lollar","sequence":"additional","affiliation":[{"name":"Department of Earth Sciences University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","unstructured":"Agency for Toxic Substances and Disease Registry. 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