{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:39:32Z","timestamp":1788248372551,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T00:00:00Z","timestamp":1404691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["RO1 AI060260"],"award-info":[{"award-number":["RO1 AI060260"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["F32 AI100350"],"award-info":[{"award-number":["F32 AI100350"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Arginine methylation is a common posttranslational modification that has far\u2010reaching cellular effects. <jats:italic>Trypanosoma brucei<\/jats:italic> is an early\u2010branching eukaryote with four characterized protein arginine methyltransferases (<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>s), one additional putative <jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>, and over 800 arginine methylated proteins, suggesting that arginine methylation has widespread impacts in this organism. While much is known about the activities of individual <jats:italic>T. brucei<\/jats:italic> PRMTs (<jats:styled-content style=\"fixed-case\">TbPRMT<\/jats:styled-content>s), little is known regarding how Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>s function together in vivo. In this study, we analyzed single and selected double Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content> knockdowns for the impact on expression of Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>s and global methylation status. Repression of Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>1 caused a decrease in asymmetric dimethylarginine and a marked increase in monomethylarginine that was catalyzed by Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>7, suggesting that Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>1 and Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>7 can compete for the same substrate. We also observed an unexpected and strong interdependence between Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>1 and Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>3 protein levels. This finding, together with the observation of similar methyl landscape profiles in Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>1 and Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>3 repressed cells, strongly suggests that these two enzymes form a functional complex. We show that corepression of Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>6\/7 synergistically impacts growth of procyclic\u2010form <jats:italic>T. brucei<\/jats:italic>. Our findings also implicate the actions of noncanonical, and as yet unidentified, <jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>s in <jats:italic>T. brucei<\/jats:italic>. Together, our studies indicate that Tb<jats:styled-content style=\"fixed-case\">PRMT<\/jats:styled-content>s display a functional interplay at multiple levels.<\/jats:p>","DOI":"10.1002\/mbo3.191","type":"journal-article","created":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T04:24:53Z","timestamp":1404707093000},"page":"595-609","source":"Crossref","is-referenced-by-count":27,"title":["Functional interplay between protein arginine methyltransferases in <i>Trypanosoma brucei<\/i>"],"prefix":"10.1002","volume":"3","author":[{"given":"Kaylen","family":"Lott","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology University at Buffalo School of Medicine Buffalo New York 14214"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University at Buffalo School of Medicine Buffalo New York 14214"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Fisk","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University at Buffalo School of Medicine Buffalo New York 14214"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle L.","family":"Tomasello","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University at Buffalo School of Medicine Buffalo New York 14214"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie K.","family":"Read","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University at Buffalo School of Medicine Buffalo New York 14214"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078015"},{"key":"e_1_2_7_3_1","first-page":"71","article-title":"SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases","volume":"4","author":"Anantharaman V.","year":"2002","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.052944"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.019885"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.12.013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838299981475"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.09.040"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.07.007"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20130747"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1977.tb00987.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep01311"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.11.010"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.525345"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.05103-11"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801021200"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807279200"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00018-10"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.022533"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006445200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.90106"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06166"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.24.5216"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R112.418574"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5070303"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.06.061"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2011.10.002"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2008-0380"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506944200"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.07.010"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq931"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2209"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.461137"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2006.08.008"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi200644c"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00133-07"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.08.015"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi.09.3"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.518803"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi002631b"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8266080"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106946108"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.27.16935"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2006.01022.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400086y"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.11.025"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.11.001"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701111104"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00002-X"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.05.004"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300186g"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(03)00071-6"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.14.3509"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.191","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.191","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.191","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.191","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.191","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:58:24Z","timestamp":1696366704000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,7]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1002\/mbo3.191"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.191","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,7]]}}}