{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T02:35:25Z","timestamp":1785551725335,"version":"3.56.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["STE-386\/10-1"],"award-info":[{"award-number":["STE-386\/10-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptomyces<\/jats:italic> sp. strain K30 induces the formation of an extracellular Lcp (latex\u2010clearing protein) during poly(<jats:italic>cis<\/jats:italic>\u20101,4\u2010isoprene) degradation. To investigate the function of this enzyme in <jats:italic>Streptomyces<\/jats:italic> sp. strain K30, the <jats:italic>lcp<\/jats:italic> gene was disrupted. This was the first time that the screening for a knock out <jats:italic>lcp<\/jats:italic> mutant of <jats:italic>Streptomyces<\/jats:italic> sp. strain K30 was successful. The resulting mutant <jats:italic>Streptomyces<\/jats:italic> sp. K30_<jats:italic>lcp<\/jats:italic>\u03a9Km exhibited reduced growth in liquid mineral salts media containing poly(<jats:italic>cis<\/jats:italic>\u20101,4\u2010isoprene) as the sole carbon and energy source. Additionally, there was no detectable Lcp activity on latex overlay agar plates. When Lcp from <jats:italic>Streptomyces<\/jats:italic> sp. strain K30 was heterologously expressed in strains TK23 and TK24 of <jats:italic>Streptomyces lividans<\/jats:italic> and a strain of <jats:italic>S. erythraea<\/jats:italic> with plasmid pIJ6021::<jats:italic>lcp<\/jats:italic>, the recombinant strains acquired the ability to cleave synthetic poly(<jats:italic>cis<\/jats:italic>\u20101,4\u2010isoprene), confirming the involvement of Lcp in initial polymer cleavage. Specific anti\u2010LcpK30 IgGs were employed in Western blot analysis to detect the secretion of Lcp in the supernatant. We have conducted an important experiment to demonstrate Lcp activity using the supernatant of these Lcp\u2010expressing strains in vitro. All three strains obviously secreted a functional Lcp, as indicated by the formation of halo. Functional testing of Lcp with different plasmids in <jats:italic>Escherichia coli<\/jats:italic> strains and <jats:italic>Pseudomonas<\/jats:italic> strains was, however, not successful.<\/jats:p>","DOI":"10.1002\/mbo3.3","type":"journal-article","created":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T07:08:41Z","timestamp":1328080121000},"page":"13-24","source":"Crossref","is-referenced-by-count":30,"title":["Importance of the latex\u2010clearing protein (Lcp) for poly(<i>cis<\/i>\u20101,4\u2010isoprene) rubber cleavage in <i>Streptomyces<\/i> sp. K30"],"prefix":"10.1002","volume":"1","author":[{"given":"Meral","family":"Yikmis","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Molekulare Mikrobiologie und Biotechnologie Westf\u00e4lische Wilhelms\u2010Universit\u00e4t M\u00fcnster  D\u201048149 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Steinb\u00fcchel","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Molekulare Mikrobiologie und Biotechnologie Westf\u00e4lische Wilhelms\u2010Universit\u00e4t M\u00fcnster  D\u201048149 M\u00fcnster Germany"},{"name":"King Abdulaziz University Jeddah  Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.6.3195-3204.2004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-68315-2_4"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.62.3.293-300.1951"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1997.1294"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.9.3680-3685.2000"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.5.2473-2478.2005"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02145-07"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb13882.x"},{"key":"e_1_2_5_10_1","first-page":"1203","article-title":"Extracellular production of Streptomyces exfoliatus poly(3\u2010hydroxybutyrate) depolymerase in Rhodococcus sp. 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