{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:11:03Z","timestamp":1791281463217,"version":"4.1.0"},"reference-count":95,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T00:00:00Z","timestamp":1452729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["MIS\u2010401170"],"award-info":[{"award-number":["MIS\u2010401170"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Burkholderia contaminans<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 shows significant antimicrobial activities against plant and animal pathogenic fungi and bacteria. The antifungal agent occidiofungin produced by\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 has great potential for development of biopesticides and pharmaceutical drugs. However, the use of\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species as biocontrol agent in agriculture is restricted due to the difficulties in distinguishing between plant growth\u2010promoting bacteria and the pathogenic bacteria. The complete\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 genome was sequenced and analyzed to find what beneficial and virulence\u2010related genes it harbors. The phylogenetic relatedness of\n                    <jats:italic>B. contaminans<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 and other 17\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species was also analyzed. To research\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14\u2032s potential virulence, the gene regions related to the antibiotic production, antibiotic resistance, and virulence were compared between\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 and other\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    genomes. The genome of\n                    <jats:italic>B. contaminans<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 was sequenced and annotated. The genomic analyses reveal the presence of multiple gene sets for antimicrobial biosynthesis, which contribute to its antimicrobial activities. BLAST results indicate that the\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 genome harbors a large number of unique regions.\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 is closely related to another plant growth\u2010promoting\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    strain\n                    <jats:italic>B. lata<\/jats:italic>\n                    383 according to the average nucleotide identity data. Moreover, according to the phylogenetic analysis, plant growth\u2010promoting species isolated from soils and mammalian pathogenic species are clustered together, respectively.\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 has multiple antimicrobial activity\u2010related genes identified from the genome, but it lacks key virulence\u2010related gene loci found in the pathogenic strains. Additionally, plant growth\u2010promoting\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species have one or more antimicrobial biosynthesis genes in their genomes as compared with nonplant growth\u2010promoting soil\u2010isolated\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species. On the other hand, pathogenic species harbor multiple virulence\u2010associated gene loci that are not present in nonpathogenic\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species. The\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    14 genome as well as\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species genome show considerable diversity. Multiple antimicrobial agent biosynthesis genes were identified in the genome of plant growth\u2010promoting species of Burkholderia. In addition, by comparing to nonpathogenic\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species, pathogenic\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species have more characterized homologs of the gene loci known to contribute to pathogenicity and virulence to plant and animals.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.333","type":"journal-article","created":{"date-parts":[[2016,1,15]],"date-time":"2016-01-15T07:53:34Z","timestamp":1452844414000},"page":"353-369","source":"Crossref","is-referenced-by-count":53,"title":["Comparative genome\u2010wide analysis reveals that\n                    <i>Burkholderia contaminans<\/i>\n                    <scp>MS<\/scp>\n                    14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis"],"prefix":"10.1002","volume":"5","author":[{"given":"Peng","family":"Deng","sequence":"first","affiliation":[{"name":"Departments of Biochemistry, Molecular Biology Entomology and Plant Pathology Mississippi State University  Mississippi state Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry, Molecular Biology Entomology and Plant Pathology Mississippi State University  Mississippi state Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonya M.","family":"Baird","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry, Molecular Biology Entomology and Plant Pathology Mississippi State University  Mississippi state Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt C.","family":"Showmaker","sequence":"additional","affiliation":[{"name":"Institute for Genomics Biocomputing and Biotechnology Mississippi State University  Mississippi state Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leif","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biology Texas A&amp;M University  College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Institute for Genomics Biocomputing and Biotechnology Mississippi State University  Mississippi state Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shien","family":"Lu","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry, Molecular Biology Entomology and Plant Pathology Mississippi State University  Mississippi state Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.10.3631-3644.2006"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2008.0017"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083779"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1537-1547.2004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1998.00473.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00997-13"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1214\/aoms\/1177699147"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00724-9"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)99733-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01218-10"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1128-1135.2004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-4-1481"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti610"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26243-0"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01750.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3052-3058.2004"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.048777-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2000.0430"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.05.006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01695-07"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2006.01894.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05231-11"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/np300678e"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408300111"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01828-09"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/016840-0"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-013-0744-x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.4.1523-1530.1997"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63991-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.01.073"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00377-11"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2010.00676.x"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00953-10"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5192-5200.2000"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/b906679a"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01230-08"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01578-12"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-9-2517"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.10.3939-3947.1998"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01556-06"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00436-07"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.059774-0"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja802966g"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.1920"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.092759.109"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp030"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-329"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(61)90408-5"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08517.x"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00349-09"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcp.0000181475.85187.ed"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi900814c"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-7-533"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1085"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03706.x"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr466"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00141604"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.3.465"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200419710"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01821-12"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1324-1333.2005"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.39.1.225"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200600393"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00414-13"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00660-13"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1021\/np3005503"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/12.2.85"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-108"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009196"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01368-13"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00420-11"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.1.248-257.1997"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63149-0"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1080\/13102818.2007.10817465"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10327-003-0096-1"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-4-41"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.19355"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2082-2087.2003"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5426-5437.2005"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02112-09"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1078\/07232020260517634"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.001123-0"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02888-0"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.15.6.1523-1524.1967"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-142"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05055-11"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.908-913.1999"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-535"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1055-7903(02)00277-4"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.333","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.333","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.333","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.333","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002%2Fmbo3.333","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.333","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T03:58:40Z","timestamp":1693713520000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,14]]},"references-count":95,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1002\/mbo3.333"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.333","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726077920.793518472","asserted-by":"object"}]},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,14]]}}}