{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:22:34Z","timestamp":1787062954386,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,10,19]],"date-time":"2016-10-19T00:00:00Z","timestamp":1476835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R21AI115183"],"award-info":[{"award-number":["1R21AI115183"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Colony opacity phase variation in\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    strain\n                    <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\n                    5075 is regulated by a reversible high\u2010frequency switch. Transposon mutagenesis was used to generate mutations that decreased the opaque to translucent switch and a gene encoding a predicted periplasmic membrane fusion component of a resistance\u2013nodulation\u2013cell division (\n                    <jats:styled-content style=\"fixed-case\">RND<\/jats:styled-content>\n                    )\u2010type efflux system was isolated. This gene was designated\n                    <jats:italic>arpA<\/jats:italic>\n                    and immediately downstream was a gene designated\n                    <jats:italic>arpB<\/jats:italic>\n                    that encodes a predicted membrane transporter of\n                    <jats:styled-content style=\"fixed-case\">RND<\/jats:styled-content>\n                    \u2010type systems. A nonpolar, in\u2010frame deletion in\n                    <jats:italic>arpA<\/jats:italic>\n                    resulted in a 70\u2010fold decrease in the opaque to translucent switch. An\n                    <jats:italic>arpB::Tc<\/jats:italic>\n                    mutant exhibited a 769\u2010fold decrease in the opaque to translucent switch. However, the translucent to opaque switch was largely unchanged in both the\n                    <jats:italic>arpA<\/jats:italic>\n                    and\n                    <jats:italic>arpB<\/jats:italic>\n                    mutants. The\n                    <jats:italic>arpA<\/jats:italic>\n                    and\n                    <jats:italic>arpB<\/jats:italic>\n                    mutants also exhibited increased surface motility in the opaque form and the\n                    <jats:italic>arpB<\/jats:italic>\n                    mutant exhibited increased susceptibility to aminoglycosides. The\n                    <jats:italic>arpA<\/jats:italic>\n                    and\n                    <jats:italic>arpB<\/jats:italic>\n                    mutants were both attenuated in a\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    model of virulence. A divergently transcribed TetR\u2010type regulator ArpR was capable of repressing the\n                    <jats:italic>\n                      arp\n                      <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\n                    <\/jats:italic>\n                    operon when this TetR regulator was overexpressed. The\n                    <jats:italic>arpR<\/jats:italic>\n                    gene was also involved in regulating the opaque to translucent switch as an in\u2010frame\n                    <jats:italic>arpR<\/jats:italic>\n                    mutation decreased this switch by 1,916\u2010fold.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.418","type":"journal-article","created":{"date-parts":[[2016,10,20]],"date-time":"2016-10-20T00:32:39Z","timestamp":1476923559000},"source":"Crossref","is-referenced-by-count":30,"title":["Multiple roles for a novel RND\u2010type efflux system in\n                    <i>Acinetobacter baumannii<\/i>\n                    AB5075"],"prefix":"10.1002","volume":"6","author":[{"given":"Kyle A.","family":"Tipton","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Emory University School of Medicine Atlanta  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Farokhyfar","sequence":"additional","affiliation":[{"name":"Research Service Atlanta VA Medical Center  Decatur GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip N.","family":"Rather","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Emory University School of Medicine Atlanta  Atlanta GA USA"},{"name":"Research Service Atlanta VA Medical Center  Decatur GA USA"},{"name":"Emory Antibiotic Resistance Center Emory University School of Medicine  Atlanta GA 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