{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:10:05Z","timestamp":1765728605015,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T00:00:00Z","timestamp":1477612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R21AI102560","R01AI080626"],"award-info":[{"award-number":["1R21AI102560","R01AI080626"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bartonella henselae<\/jats:italic> is a gram\u2010negative zoonotic bacterium that causes infections in humans including endocarditis and bacillary angiomatosis. <jats:italic>B.\u00a0henselae<\/jats:italic> has been shown to grow as large aggregates and form biofilms in vitro. The aggregative growth and the angiogenic host response requires the trimeric autotransporter adhesin BadA. We examined the transcriptome of the Houston\u20101 strain of <jats:italic>B.\u00a0henselae<\/jats:italic> using <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq revealing nine novel, highly\u2010expressed intergenic transcripts (<jats:italic>Bartonella<\/jats:italic> regulatory transcript, Brt1\u20109). The Brt family of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s is unique to the genus <jats:italic>Bartonella<\/jats:italic> and ranges from 194 to 203 nucleotides with high homology and stable predicted secondary structures. Immediately downstream of each of the nine <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> genes is a helix\u2010turn\u2010helix <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010binding protein (transcriptional regulatory protein, Trp1\u20109) that is poorly transcribed under the growth conditions used for <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq. Using knockdown or overexpressing strains, we show a role of both the Brt1 and Trp1 in the regulation of <jats:italic>badA<\/jats:italic> and also in biofilm formation. Based on these data, we hypothesize that Brt1 is a <jats:italic>trans<\/jats:italic>\u2010acting <jats:styled-content style=\"fixed-case\">sRNA<\/jats:styled-content> that also serves as a <jats:italic>cis<\/jats:italic>\u2010acting riboswitch to control the expression of <jats:italic>badA<\/jats:italic>. This family of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s together with the downstream Trp <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010binding proteins represents a novel coordinated regulatory circuit controlling expression of virulence\u2010associated genes in the bartonellae.<\/jats:p>","DOI":"10.1002\/mbo3.420","type":"journal-article","created":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T07:36:43Z","timestamp":1477640203000},"source":"Crossref","is-referenced-by-count":13,"title":["A family of genus\u2010specific <scp>RNA<\/scp>s in tandem with <scp>DNA<\/scp>\u2010binding proteins control expression of the <i>badA<\/i> major virulence factor gene in <i>Bartonella henselae<\/i>"],"prefix":"10.1002","volume":"6","author":[{"given":"Nhan","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronan K.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Ohio University Athens OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Microbiology College of Arts and Sciences University of South Florida Tampa FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsey N.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Microbiology College of Arts and Sciences University of South Florida Tampa FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gael","family":"Nicolas","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Morsani College of Medicine University of South Florida Tampa FL 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