{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T20:45:07Z","timestamp":1785876307727,"version":"3.56.0"},"reference-count":71,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T00:00:00Z","timestamp":1498003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004441","name":"Styrelsen f\u00f6r Internationellt Utvecklingssamarbete","doi-asserted-by":"publisher","award":["51000131"],"award-info":[{"award-number":["51000131"]}],"id":[{"id":"10.13039\/100004441","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endophytic and plant\u2010associated bacteria were isolated from plants and rhizoplane soil of naturally grown Brachiaria grasses at International Livestock Research Institute in Nairobi, Kenya. Eighty\u2010four bacterial strains were isolated from leaf tissues, root tissues, and rhizoplane soil on nutrient agar and 869 media. All bacterial strains were identified to the lowest possible taxonomic unit using 16S r<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> primers and were characterized for the production of Indole\u20103\u2010acetic acid, hydrogen cyanide, and <jats:styled-content style=\"fixed-case\">ACC<\/jats:styled-content> deaminase; phosphate solubilization; siderophore production; antifungal properties; and plant biomass production. The 16S r<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010based identification grouped these 84 bacterial strains into 3 phyla, 5 classes, 8 orders, 12 families, 16 genera, and 50 unique taxa. The four most frequently isolated genera were <jats:italic>Pseudomonas<\/jats:italic> (23), <jats:italic>Pantoea<\/jats:italic> (17), <jats:italic>Acinetobacter<\/jats:italic> (9), and <jats:italic>Enterobacter<\/jats:italic> (8). The functional characterization of these strains revealed that 41 of 84 strains had a minimum of three plant beneficial properties. Inoculation of maize seedlings with <jats:italic>Acinetobacter<\/jats:italic> spp., <jats:italic>Microbacterium<\/jats:italic> spp., <jats:italic>Pectobacterium<\/jats:italic> spp., <jats:italic>Pseudomonas<\/jats:italic> spp., and <jats:italic>Enterobacter<\/jats:italic> spp. showed positive effects on seedling biomass production. The ability of Brachiaria grasses to host genetically diverse bacteria, many of them with multiple plant growth\u2010promoting attributes, might have contributed to high biomass production and adaptation of Brachiaria grasses to drought and low fertility soils.<\/jats:p>","DOI":"10.1002\/mbo3.497","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T04:12:57Z","timestamp":1498104777000},"source":"Crossref","is-referenced-by-count":17,"title":["Brachiaria Grasses (<i>Brachiaria spp<\/i>.) harbor a diverse bacterial community with multiple attributes beneficial to plant growth and development"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0951-9023","authenticated-orcid":false,"given":"Collins","family":"Mutai","sequence":"first","affiliation":[{"name":"Biosciences eastern and central Africa\u2010International Livestock Research Institute (BecA\u2010ILRI) Hub  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce","family":"Njuguna","sequence":"additional","affiliation":[{"name":"Biosciences eastern and central Africa\u2010International Livestock Research Institute (BecA\u2010ILRI) Hub  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8930-1384","authenticated-orcid":false,"given":"Sita","family":"Ghimire","sequence":"additional","affiliation":[{"name":"Biosciences eastern and central Africa\u2010International Livestock Research Institute (BecA\u2010ILRI) Hub  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,21]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Livestock Production in Nigeria: Our Commission and Omission","author":"Ademosun A. 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