{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:56:39Z","timestamp":1787702199899,"version":"build-2784847793"},"reference-count":87,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:00:00Z","timestamp":1502323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31601574"],"award-info":[{"award-number":["31601574"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Genetic information about <jats:italic>Enterococcus hirae<\/jats:italic> is limited, a feature that has compromised our understanding of these clinically challenging bacteria. In this study, comparative analysis was performed of <jats:italic>E.\u00a0hirae<\/jats:italic> R17, a daptomycin\u2010resistant strain isolated from pork purchased from a retail market in Beijing, China, and three other enterococcal genomes (<jats:italic>Enterococcus\u00a0faecium <\/jats:italic><jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>,<jats:italic> Enterococcus\u00a0faecalis<\/jats:italic> V583, and <jats:italic>E.\u00a0hirae <\/jats:italic><jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content><jats:sup>\u2122<\/jats:sup>9790). Some 1,412 genes were identified that represented the core genome together with an additional 139 genes that were specific to <jats:italic>E.\u00a0hirae<\/jats:italic> R17. The functions of these R17 strain\u2010specific coding sequences relate to the <jats:styled-content style=\"fixed-case\">COG<\/jats:styled-content>s categories of <jats:italic>carbohydrate transport and metabolism<\/jats:italic> and <jats:italic>transcription<\/jats:italic>, a finding that suggests the carbohydrate utilization capacity of <jats:italic>E.\u00a0hirae<\/jats:italic> R17 may be more extensive when compared with the other three bacterial species (spp.). Analysis of genomic islands and virulence genes highlighted the potential that horizontal gene transfer played as a contributor of variations in pathogenicity in this isolate. Drug\u2010resistance gene prediction and antibiotic susceptibility testing indicated <jats:italic>E.\u00a0hirae<\/jats:italic> R17 was resistant to several antimicrobial compounds, including bacitracin, ciprofloxacin, daptomycin, erythromycin, and tetracycline, thereby limiting chemotherapeutic treatment options. Further, tolerance to biocides and metals may confer a phenotype that facilitates the survival and adaptation of this isolate against food preservatives, disinfectants, and antibacterial coatings. The genomic plasticity, mediated by <jats:styled-content style=\"fixed-case\">IS<\/jats:styled-content> elements, transposases, and tandem repeats, identified in the <jats:italic>E.\u00a0hirae<\/jats:italic> R17 genome may support adaptation to new environmental niches, such as those that are found in hospitalized patients. A predicted transmissible plasmid, <jats:styled-content style=\"fixed-case\">pRZ<\/jats:styled-content>1, was found to carry several antimicrobial determinants, along with some predicted pathogenic genes. These data supported the previously determined phenotype confirming that the foodborne <jats:italic>E.\u00a0hirae<\/jats:italic> R17 is a multidrug\u2010resistant pathogenic bacterium with evident genome plasticity and environmental adaptability.<\/jats:p>","DOI":"10.1002\/mbo3.514","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T01:52:50Z","timestamp":1502416370000},"source":"Crossref","is-referenced-by-count":13,"title":["Genomic insights into the pathogenicity and environmental adaptability of <i>Enterococcus hirae<\/i> R17 isolated from pork offered for retail sale"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5451-2111","authenticated-orcid":false,"given":"Zixin","family":"Peng","sequence":"first","affiliation":[{"name":"Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment  Beijing China"},{"name":"State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtao","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9amus","family":"Fanning","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment  Beijing China"},{"name":"UCD\u2010Centre for Food Safety School of Public Health Physiotherapy and Sports Science University College Dublin  Belfield Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.11.067"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmmcr.0.001214"},{"key":"e_1_2_7_4_1","first-page":"1534","article-title":"Enterococcus faecalis from Food, Clinical Specimens, and Oral Sites: Prevalence of Virulence Factors in Association with Biofilm Formation","volume":"6","author":"Anderson A. 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