{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:00:15Z","timestamp":1790614815522,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ixodidae ticks are vectors and reservoirs of several species of rickettsiae, and tick\u2010borne rickettsioses are reported worldwide. This study was aimed to verify the distribution of spotted fever group rickettsiae associated with ticks in a wild environment, the National Park of Gargano, where there is proximity between wild and domestic animals, and which is within an endemic area for rickettsiosis. Ticks were collected from animals or vegetation, morphologically identified and tested by a\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    targeting the 17kDa gene, and by a loop\u2010mediated isothermal amplification (\n                    <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>\n                    ) targeting\n                    <jats:italic>ompB<\/jats:italic>\n                    gene. Out of 34 tested tick pools, 2 from\n                    <jats:italic>Dermacentor marginatus,<\/jats:italic>\n                    1 from\n                    <jats:italic>Ixodes ricinus,<\/jats:italic>\n                    and 1 from\n                    <jats:italic>Rhipicephalus turanicus<\/jats:italic>\n                    resulted positive. Nucleotide sequences of amplicons showed high similarity with sequences from\n                    <jats:italic>Rickettsia slovaca<\/jats:italic>\n                    ,\n                    <jats:italic>Rickettsia raoultii<\/jats:italic>\n                    ,\n                    <jats:italic>Rickettsia helvetica,<\/jats:italic>\n                    and\n                    <jats:italic>Rickettsia felis<\/jats:italic>\n                    . The overall calculated infection rate was 26.19 per 1,000, while it rose up to 107.77 when only\n                    <jats:italic>D. marginatus<\/jats:italic>\n                    was considered. The results highlight the association among\n                    <jats:italic>Ri. slovaca<\/jats:italic>\n                    ,\n                    <jats:italic>Ri. raoultii<\/jats:italic>\n                    ,\n                    <jats:italic>D. marginatus<\/jats:italic>\n                    and wild boars from which infected ticks were collected. Finally, the study shows the low efficacy of the previously described\n                    <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>\n                    method for the detection of\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp., when compared to\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    , making urgent the development of most effective\n                    <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>\n                    protocols.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.527","type":"journal-article","created":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T00:09:09Z","timestamp":1508371749000},"source":"Crossref","is-referenced-by-count":15,"title":["Spotted fever group rickettsiae associated with ixodid ticks in wild environment in Southern Italy"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2627-0669","authenticated-orcid":false,"given":"Donato Antonio","family":"Raele","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Galante","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9342-9516","authenticated-orcid":false,"given":"Nicola","family":"Pugliese","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna La","family":"Salandra","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Assunta","family":"Cafiero","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2012.1201"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.04.009"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1103.040812"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2005.5.420"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00138.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-016-0070-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-2-S1-S8"},{"key":"e_1_2_6_9_1","first-page":"518","article-title":"Rickettsia conorii israelensis in Rhipicephalus sanguineus ticks, Sardinia, Italy","volume":"4","author":"Chisu V.","year":"2013","journal-title":"Ticks Tick Borne Diseases"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1374.024"},{"key":"e_1_2_6_11_1","volume-title":"A guide of identification of species","author":"Estrada\u2010Pe\u00f1a A.","year":"2014"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07357.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04528.x"},{"key":"e_1_2_6_14_1","first-page":"446","article-title":"A molecular survey of Rickettsia felis in fleas from cats and dogs in Sicily (Southern Italy)","volume":"5","author":"Giudice E.","year":"2014","journal-title":"Ticks Tick Borne Diseases"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn201"},{"key":"e_1_2_6_16_1","first-page":"89","article-title":"The role of particular tick developmental stages in the circulation of tick\u2010borne pathogens affecting humans in Central Europe. 3 Rickettsiae.","volume":"62","author":"Karbowiak G.","year":"2016","journal-title":"Annals of Parasitology"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-016-0921-z"},{"key":"e_1_2_6_18_1","first-page":"284","article-title":"Characterization of spotted fever group Rickettsiae in ticks from a city park of Rome, Italy","volume":"51","author":"Mancini F.","year":"2015","journal-title":"Annali dell Istituto Superiore di Sanita"},{"key":"e_1_2_6_19_1","volume-title":"Fauna d'Italia","author":"Manilla G.","year":"1998"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2013.07.002"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2012.10.007"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2012.10.035"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-328"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-254"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.719-756.2005"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2001.00200.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1507.081449"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-1128-3"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1534-1"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2015.09.009"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2015.09.011"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004789"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1086\/340100"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1128\/jcm.34.9.2058-2065.1996","article-title":"Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR\u2010amplified DNA of the gene encoding the protein rOmpA","volume":"34","author":"Roux V.","year":"1996","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1948-7134.2013.12022.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1405.070976"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1603\/033.046.0636"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-66"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.530-534.1990"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-7-6"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02735-5"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.527","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:52:13Z","timestamp":1772988733000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,18]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/mbo3.527"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.527","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,18]]},"article-number":"e00527"}}