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Therefore, the antibacterial activities of chitosan against four environmental isolates of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    from soil in Khon Kaen, Thailand, were investigated. Antibacterial activities were assessed by a plate count technique after treatment with 0.2, 0.5, 1, 2 or 5\u00a0mg\u00a0ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    chitosan for 0, 24 and 48\u00a0hr. Chitosan at 5\u00a0mg\u00a0ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    completely killed all four\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    isolates within 24\u00a0hr, whilst 2\u00a0mg\u00a0ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    chitosan lowered the viability of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    by 20% within the same time span. Chitosan may act by disruption of the cell membrane, releasing intracellular components that can be detected spectrophotometrically at 260 and 280\u00a0nm. Transmission electron microscopy inspection of chitosan\u2010treated\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    revealed damage to the bacterial membranes. This study demonstrated the effective antibacterial activity by chitosan against\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    . Chitosan causes disruption of the bacterial cell membrane, release of intracellular constituents and cell death. This study revealed the inhibitory potential of chitosan for mitigating\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    occurrences.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.534","type":"journal-article","created":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T00:59:19Z","timestamp":1511744359000},"source":"Crossref","is-referenced-by-count":22,"title":["Antibacterial activity of chitosan against\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1002","volume":"7","author":[{"given":"Watcharaporn","family":"Kamjumphol","sequence":"first","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Khon Kaen University  Khon Kaen Thailand"},{"name":"Melioidosis Research Center Khon Kaen University  Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3717-5889","authenticated-orcid":false,"given":"Pisit","family":"Chareonsudjai","sequence":"additional","affiliation":[{"name":"Melioidosis Research Center Khon Kaen University  Khon Kaen Thailand"},{"name":"Department of Environmental Science Faculty of Science Khon Kaen University  Khon Kaen Thailand"},{"name":"Biofilm Research Group Khon Kaen University  Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7176-2924","authenticated-orcid":false,"given":"Sorujsiri","family":"Chareonsudjai","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Khon Kaen University  Khon Kaen Thailand"},{"name":"Melioidosis Research Center Khon Kaen University  Khon Kaen Thailand"},{"name":"Biofilm Research Group Khon Kaen University  Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.04.098"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-016-0302-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2008.06.009"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02930965"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2014.08.005"},{"key":"e_1_2_7_8_1","first-page":"932","article-title":"Relationship between antibacterial activity of chitosan and surface characteristics of cell wall","volume":"25","author":"Chung Y. 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