{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T10:19:51Z","timestamp":1778926791621,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T00:00:00Z","timestamp":1514419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minist\u00e8re de lenseignement sup\u00e9rieur et de la recherche"},{"name":"Laboratoire de recherche microorganismes et pathologies humaines"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two techniques based on <jats:italic>ompA<\/jats:italic> amplification of <jats:italic>Chlamydia trachomatis<\/jats:italic> were compared, being reverse hybridization (<jats:styled-content style=\"fixed-case\">RHM<\/jats:styled-content>) and <jats:italic>ompA<\/jats:italic> sequencing (<jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content>), to investigate the concordance between them and to study the epidemiological relevance of each method. In addition, phylogenetic analysis was performed on the <jats:italic>ompA<\/jats:italic> sequences. One hundred and seven <jats:italic>C. trachomatis<\/jats:italic> positive samples from Tunisian patients and female sex workers were analyzed using both the <jats:styled-content style=\"fixed-case\">RHM<\/jats:styled-content> and <jats:italic>ompA<\/jats:italic> sequencing. The overall genovar distribution obtained with both techniques was very similar. The <jats:styled-content style=\"fixed-case\">RHM<\/jats:styled-content> identified nine genovars, being B, D, E, F, G, H, I, J and K, where B, I, J, and K were only found in mixed infections versus 7 types for the <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> being D, E, F, G, H, I, and K. The agreement between both typing techniques was 87.8%. Both methods showed that genovar E was the most predominant type. In 24.3% of the analyzed samples, mixed infections were detected. In 96.1% of these, the genovar identified by <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> was also detected using the <jats:styled-content style=\"fixed-case\">RHM<\/jats:styled-content>. O<jats:italic>mpA<\/jats:italic> sequencing allowed determination of six genovar types that could not be typed using <jats:styled-content style=\"fixed-case\">RHM<\/jats:styled-content>. The analyses of <jats:italic>ompA<\/jats:italic> nucleotide variation in the 107 clinical specimens detected <jats:italic>ompA<\/jats:italic> genovar variants with distinct <jats:italic>ompA<\/jats:italic> mutational patterns for types D2, G1, G2, and H1. In conclusion, <jats:styled-content style=\"fixed-case\">RHM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> showed a high agreement in <jats:italic>C. trachomatis<\/jats:italic> genotyping results with each having their specific benefits.<\/jats:p>","DOI":"10.1002\/mbo3.549","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T05:36:48Z","timestamp":1514439408000},"source":"Crossref","is-referenced-by-count":4,"title":["Comparison of reverse hybridization and <i>ompA<\/i> sequencing methods applied on <i>Chlamydia trachomatis<\/i> strains from Tunisia"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9151-8056","authenticated-orcid":false,"given":"Houda","family":"Gharsallah","sequence":"first","affiliation":[{"name":"Department of Microbiology and research laboratory \u201cMicroorganismes et Pathologies Humaines\u201d Habib Bourguiba university hospital Medical School of Sfax University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olfa","family":"Frikha\u2010Gargouri","sequence":"additional","affiliation":[{"name":"Biopesticides Laboratory Centre of Biotechnology of Sfax University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinier J.","family":"Bom","sequence":"additional","affiliation":[{"name":"Public Health Laboratory The Netherlands Condomerie Cluster Infectious Diseases Public Health Service Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adnene","family":"Hammami","sequence":"additional","affiliation":[{"name":"Department of Microbiology and research laboratory \u201cMicroorganismes et Pathologies Humaines\u201d Habib Bourguiba university hospital Medical School of Sfax University of Sfax  Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia M.","family":"Bruisten","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Public Health Service of Amsterdam (GGD Amsterdam)  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10688"},{"issue":"8","key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.1128\/JCM.00128-11","article-title":"Evaluation of high\u2010resolution typing methods for Chlamydia trachomatis in samples from heterosexual couples","volume":"49","author":"Bom R. 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