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Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and two from dental waste (days 0 and 180). Slide culture, microscopy, antifungal drug susceptibility, intersimple sequence repeat analysis, and amplification and sequencing of internal transcribed spacer regions were performed. Yeast strains were recovered from all waste workers' sites, including professional clothes, and from waste. Antifungal susceptibility testing demonstrated that some yeast recovered from employees and waste exhibited nonsusceptible profiles. The dendrogram demonstrated the presence of three major clusters based on similarity matrix and UPGMA grouping method. Two branches displayed 100% similarity: three strains of <jats:italic>Candida guilliermondii<\/jats:italic> isolated from different employees, working in opposite work shifts, and from diverse sites grouped in one part of branch 1 and cluster 3 that included two samples of <jats:italic>Candida albicans<\/jats:italic> recovered from waste and the hand of one waste worker. The results suggested the possibility of cross\u2010contamination from dental waste to waste workers and reinforce the need of training programs focused on better waste management routines.<\/jats:p>","DOI":"10.1002\/mbo3.554","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T06:26:37Z","timestamp":1514269597000},"source":"Crossref","is-referenced-by-count":4,"title":["Investigating cross\u2010contamination by yeast strains from dental solid waste to waste\u2010handling workers by DNA sequencing"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6957-5329","authenticated-orcid":false,"given":"Cristina Dutra","family":"Vieira","sequence":"first","affiliation":[{"name":"Laboratory of Oral Microbiology and Anaerobes Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaysa Leite","family":"Tagliaferri","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Microbiology and Anaerobes Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Auxiliadora Roque","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Microbiology and Anaerobes Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida","family":"de Resende\u2010Stoianoff","sequence":"additional","affiliation":[{"name":"Mycology Laboratory Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Assuncao","family":"Holanda","sequence":"additional","affiliation":[{"name":"Mycology Laboratory Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"},{"name":"Parasite Biology Laboratory CEUMA University \u2010 Rua dos Castanheiros  S\u00e3o Lu\u00eds Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Furtado Ferreira","family":"de Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Mycology Laboratory Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Prazeres","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Microbiology and Anaerobes Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone Gon\u00e7alves","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Microbiology and Anaerobes Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz","family":"de Mac\u00eado Farias","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Microbiology and Anaerobes Department of Microbiology Institute of Biological Sciences Federal University of Minas Gerais \u2010 Avenida Ant\u00f4nio Carlos  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652015000700011"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.6061\/clinics\/2012(10)18","article-title":"First report of a clinical isolate of Candida haemulonii in Brazil","volume":"67","author":"Almeida J. 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