{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:53:04Z","timestamp":1790761984008,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T00:00:00Z","timestamp":1516320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T00:00:00Z","timestamp":1516320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2018,6]]},"abstract":"Abstract<\/jats:title>\n \n Vibrio alginolyticus<\/jats:italic>\n (\n V.\u00a0alginolyticus<\/jats:italic>\n ) is a common pathogen for humans and marine aquatic animals. Vibriosis of marine aquatic animals, caused by\n V.\u00a0alginolyticus<\/jats:italic>\n , has become more prevalent globally in recent years. Hence, a safe and effective vaccine is urgently needed for the control of this disease. Here, the strain 16\u20103 isolated from the large yellow croaker (\n Larimichthys crocea<\/jats:italic>\n ) suffered from canker was identified as\n V.\u00a0alginolyticus<\/jats:italic>\n based on morphological, biochemical, and 16S\n rDNA<\/jats:styled-content>\n sequencing analysis. Then, recombinant temperature\u2010controlled lysis plasmid\n pBV<\/jats:styled-content>\n 220\u2010lysisE was electroporated into the strain 16\u20103 to generate\n V.\u00a0alginolyticus<\/jats:italic>\n bacterial ghosts (Va\n BG<\/jats:styled-content>\n s) by inducing lysis gene E expression, and the safety and immune effects of Va\n BG<\/jats:styled-content>\n s were further investigated in mice and large yellow croaker. The results showed that Va\n BG<\/jats:styled-content>\n s were as safe as formalin\u2010killed\n V.\u00a0alginolyticus<\/jats:italic>\n cells (\n FKC<\/jats:styled-content>\n ) to mice and fish. Compared with\n FKC<\/jats:styled-content>\n and\n PBS<\/jats:styled-content>\n groups, significant elevations of the serum agglutinating antibody titer, serum bactericidal activity, lymphocyte proliferative responses, and levels of four different cytokines (Th1 type:\n IL<\/jats:styled-content>\n \u20102,\n TNF<\/jats:styled-content>\n \u2010\u03b1; Th2 type:\n IL<\/jats:styled-content>\n \u20104 and\n IL<\/jats:styled-content>\n \u20106) in serum were detected in the Va\n BG<\/jats:styled-content>\n s group, indicating that a Th1\/Th2\u2010mediated mixed immune response was elicited by the Va\n BG<\/jats:styled-content>\n s. More importantly, after challenged with the parent strain 16\u20103, Va\n BG<\/jats:styled-content>\n s\u2010vaccinated mice and fish showed higher protection than\n FKC<\/jats:styled-content>\n \u2010vaccinated mice, the relative percent of survival (\n RPS<\/jats:styled-content>\n ) being 60%, 66.7% and 40%, respectively. Taken together, this is the first demonstration that the newly constructed\n V.\u00a0alginolyticus<\/jats:italic>\n ghosts may be developed as a safe and effective vaccine against\n V.\u00a0alginolyticus<\/jats:italic>\n infection in aquaculture.\n <\/jats:p>","DOI":"10.1002\/mbo3.576","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T00:09:03Z","timestamp":1516320543000},"source":"Crossref","is-referenced-by-count":42,"title":["Identification of fish source\n Vibrio alginolyticus<\/i>\n and evaluation of its bacterial ghosts vaccine immune effects"],"prefix":"10.1002","volume":"7","author":[{"given":"Ji","family":"Cao","sequence":"first","affiliation":[{"name":"Key Laboratory of Zoonoses College of Animal Science and Technology Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonoses College of Animal Science and Technology Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonoses College of Animal Science and Technology Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonoses College of Animal Science and Technology Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonoses College of Animal Science and Technology Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1634-6359","authenticated-orcid":false,"given":"Jinnian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonoses College of Animal Science and Technology Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,19]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"447","article-title":"Potency testing of fish vaccines international symposium on fish biologics: Serodiagnostics and vaccinaes","volume":"49","author":"Amend D.","year":"1981","journal-title":"Developments in Biological Standardization"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1556\/AVet.59.2011.1.13"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02617"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02206"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2009.10.003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-44"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201789"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.09.014"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2013.08.008"},{"key":"e_1_2_8_11_1","first-page":"BR362","article-title":"T cell\u2010specific immune response induced by bacterial ghosts","volume":"10","author":"Felnerova D.","year":"2004","journal-title":"Medical Science Monitor International Medical Journal of Experimental & Clinical Research"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.98-104.2005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(00)00298-4"},{"key":"e_1_2_8_14_1","first-page":"509","article-title":"Evaluation of safe attenuated Vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis","volume":"4","author":"Idris N. 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