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The autoinducer 2 (<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102) quorum sensing system is widely present in many species of gram\u2010negative and gram\u2010positive bacteria and has been proposed to be involved in interspecies communication. In clinical <jats:styled-content style=\"fixed-case\">APEC<\/jats:styled-content> strains, whether or not <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102 affects the expression of antibiotic\u2010related genes has not been reported. In this study, we have reported that exogenous <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102 increase the susceptibility of <jats:styled-content style=\"fixed-case\">APEC<\/jats:styled-content> strains to trimethoprim\u2010sulfamethoxazole (<jats:styled-content style=\"fixed-case\">SXT<\/jats:styled-content>) in a folate synthesis\u2010dependent pathway but not in the LsrR\u2010dependent manner. Our results further explained that exogenous <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102 can down regulate the transcription of the folate synthetase encoding genes <jats:italic>folA<\/jats:italic> and <jats:italic>folC<\/jats:italic>, and the folate synthesis\u2010related genes <jats:italic>luxS, metE,<\/jats:italic> and <jats:italic>metH<\/jats:italic>. Gel shift assays confirmed that LsrR, the <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102 receptor, did not bind to the promoters of <jats:italic>folA<\/jats:italic> and <jats:italic>folC<\/jats:italic>, suggesting that exogenous <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102 might influence folate metabolism by a feedback inhibition effect but not in the LsrR\u2010dependent pathway. This study might provide further information in the search for potential drug targets for prophylaxis of avian colibacillosis and for auxiliary antibiotics in the treatment of avian colibacillosis.<\/jats:p>","DOI":"10.1002\/mbo3.582","type":"journal-article","created":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T05:52:51Z","timestamp":1518155571000},"source":"Crossref","is-referenced-by-count":17,"title":["Autoinducer2 affects trimethoprim\u2010sulfamethoxazole susceptibility in avian pathogenic <i>Escherichia coli<\/i> dependent on the folate synthesis\u2010associate pathway"],"prefix":"10.1002","volume":"7","author":[{"given":"Lumin","family":"Yu","sequence":"first","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunmei","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingtian","family":"Ni","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Anhui Key Laboratory of Zoonoses Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Shang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5644-6613","authenticated-orcid":false,"given":"Ting","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.163.4.402"},{"issue":"3","key":"e_1_2_7_3_1","first-page":"303","article-title":"Antimicrobial resistance of Escherichia coli isolated from chickens with colibacillosis","volume":"4","author":"Aggad H.","year":"2010","journal-title":"Global Veterinaria"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm124"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00546-09"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2002.221.411"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(99)00025-9"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00749-3"},{"issue":"8","key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1128\/jcm.35.8.2184-2185.1997","article-title":"Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain","volume":"35","author":"Blanco J. 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