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Of the pathogenic\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species,\n                    <jats:italic>N. seriolae<\/jats:italic>\n                    is known to cause disease in cultured fish, resulting in major economic loss. We isolated two\n                    <jats:italic>N. seriolae<\/jats:italic>\n                    strains,\n                    <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>\n                    \u201014008 and\n                    <jats:styled-content style=\"fixed-case\">EM<\/jats:styled-content>\n                    15050, from diseased fish and sequenced their genomes using the PacBio sequencing platform. To identify their genomic features, we compared their genomes with those of other\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species. Phylogenetic analysis showed that\n                    <jats:italic>N. seriolae<\/jats:italic>\n                    shares a common ancestor with a putative human pathogenic\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species. Moreover,\n                    <jats:italic>N. seriolae<\/jats:italic>\n                    strains were phylogenetically divided into four clusters according to host fish families. Through genome comparison, we observed that the putative pathogenic\n                    <jats:italic>Nocardia<\/jats:italic>\n                    strains had additional genes for iron acquisition. Dozens of antibiotic resistance genes were detected in the genomes of\n                    <jats:italic>N. seriolae<\/jats:italic>\n                    strains; most of the antibiotics were involved in the inhibition of the biosynthesis of proteins or cell walls. Our results demonstrated the virulence features and antibiotic resistance of fish pathogenic\n                    <jats:italic>N. seriolae<\/jats:italic>\n                    strains at the genomic level. These results may be useful to develop strategies for the prevention of fish nocardiosis.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.656","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T00:16:01Z","timestamp":1534464961000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Genomic characterization of\n                    <i>Nocardia seriolae<\/i>\n                    strains isolated from diseased fish"],"prefix":"10.1002","volume":"8","author":[{"given":"Hyun\u2010Ja","family":"Han","sequence":"first","affiliation":[{"name":"Pathology Research Division National Institute of Fisheries Science  Busan Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Jung","family":"Kwak","sequence":"additional","affiliation":[{"name":"ChunLab Inc.  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