{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:57:00Z","timestamp":1780588620030,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T00:00:00Z","timestamp":1361145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sepsis is associated with high morbidity and mortality rates worldwide. Rapid and reliable diagnostic methods are needed for efficient and evidence\u2010based treatment of septic patients. Recently, new molecular tools have emerged to complement the conventional culture\u2010based diagnostic methods. In this study, we used spiked whole blood samples to evaluate together two ready\u2010to\u2010use molecular solutions for the detection of sepsis\u2010causing bacteria. We spiked whole blood with bacterial species relevant in sepsis and extracted bacterial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> with the NorDiag Arrow device, using the SelectNA Blood pathogen <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> isolation kit. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extracts were analyzed by the polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>)\u2010 and microarray\u2010based Prove\u2010it\u2122 Bone and Joint assay, resulting in correctly identified bacterial species with detection limits of 11\u2013600\u00a0colony\u2010forming unit\/mL (<jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/mL). To understand the recovery losses of bacterial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> during the sample preparation step and the capability of the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010 and microarray\u2010based platform to respond to the sensitivity requirements, we also determined the analytical sensitivity of the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and microarray platform to be 1\u201321 genome equivalents for the tested bacterial species. In addition, the inclusivity of the Prove\u2010it\u2122 Bone and Joint assay was demonstrated with methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">MRSA<\/jats:styled-content>) clones carrying <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content><jats:italic>mec<\/jats:italic> types I, II, IV, or V and a nontypable SCC<jats:italic>mec<\/jats:italic> type. The proof\u2010of\u2010concept for accurate multiplex pathogen and antibacterial resistance marker detection from spiked whole blood samples was demonstrated by the selective bacterial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction method combined with the high\u2010throughput <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010 and microarray\u2010based platform. Further investigations are needed to study the promising potential of the concept for sensitive, semi\u2010automated identification of sepsis\u2010causing pathogens directly from whole blood.<\/jats:p>","DOI":"10.1002\/mbo3.69","type":"journal-article","created":{"date-parts":[[2013,2,19]],"date-time":"2013-02-19T07:34:53Z","timestamp":1361259293000},"page":"284-292","source":"Crossref","is-referenced-by-count":12,"title":["Assessment of a semi\u2010automated protocol for multiplex analysis of sepsis\u2010causing bacteria with spiked whole blood samples"],"prefix":"10.1002","volume":"2","author":[{"given":"Sanna","family":"Laakso","sequence":"first","affiliation":[{"name":"Mobidiag Ltd. Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minna","family":"M\u00e4ki","sequence":"additional","affiliation":[{"name":"Mobidiag Ltd. Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc11202"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/JCM.38.1.345-350.2000","article-title":"Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR","volume":"1","author":"Al\u2010Sould W. A.","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.032144"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq515"},{"key":"e_1_2_6_6_1","first-page":"44","article-title":"High performance DNA clean\u2010up","volume":"3","author":"Disque C.","year":"2004","journal-title":"Eur. Biotech. 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D. M. P.Heron S. L.Murphy andK. D.Kochanek.2007.Deaths: final data for 2004. National Vital Statistics Report. National Center for Health Statistics. National Vital Statistics Reports55:19."},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.07.011"},{"key":"e_1_2_6_20_1","unstructured":"M\u00fchl H. S.Keim N.Murphy andM. G.Lorenz.2011.Automated extraction of microbial DNA from whole blood for the universal PCR detection of pathogens. Poster 11th ASM meeting 21\u201024 May 2011 New Orleans. Referred: 20 July 2012."},{"key":"e_1_2_6_21_1","unstructured":"NorDiag ASA NorDiag Arrow with Molzym Blood pathogen cartridges provides an automated solution for concentrating bacterial and fungal nucleic acids in blood. Application Note AN\u201019\u201010. Available athttp:\/\/www.nordiag.com. 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