{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:20:39Z","timestamp":1790792439817,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The emergence of antimicrobial resistance (AMR) and extended\u2010spectrum beta\u2010lactamase (ESBL)\u2010producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (ESBL\u2010EC) in seafood represents a significant public health concern. In this study, we screened 102 raw seafood samples, comprising shrimp (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200942), sea fish (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200936), and crabs (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200924), to detect ESBL\u2010EC.\n                    <jats:italic>E. coli<\/jats:italic>\n                    was isolated and identified through culture\u2010based methods, staining procedures, biochemical assays, and polymerase chain reaction (PCR) analysis. The AMR properties of\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates were evaluated using the disc diffusion test, while ESBL\u2010EC was identified phenotypically through the double\u2010disc synergy test and confirmed at the genetic level using PCR. PCR analysis revealed that 42.2% (43\/102) of the samples were contaminated with\n                    <jats:italic>E. coli<\/jats:italic>\n                    , with sea fish showing the highest (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) prevalence (63.9%, 23\/36), followed by crabs (37.5%, 9\/24) and shrimp (26.2%, 11\/42). All the isolates exhibited phenotypic resistance to ampicillin, followed by ceftazidime (95.3%), ciprofloxacin (55.8%), azithromycin (39.5%), cefotaxime (37.2%), and streptomycin (16.3%). Notably, 69.8% (30\/43) of\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates exhibited phenotypically multidrug resistance. Moreover, 18.6% (8\/43) of the isolates showed ESBL\u2010producing characteristics, higher in shrimp than in sea fish and crabs. ESBL\u2010related gene,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    , was detected in 75% (6\/8),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV<\/jats:sub>\n                    in 87.5% (7\/8), and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    in 50% (4\/8) of the ESBL\u2010EC isolates. Regular surveillance of seafood for antimicrobial\u2010resistant bacteria, particularly ESBL\u2010producing strains, is recommended due to their potential public health implications.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70023","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T06:39:32Z","timestamp":1751006372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Detection and Characterization of Extended\u2010Spectrum Beta\u2010Lactamase\u2010Producing\n                    <i>Escherichia coli<\/i>\n                    in Raw Seafood From the Coastal Area of Bangladesh"],"prefix":"10.1002","volume":"14","author":[{"given":"Zannatul","family":"Firdous","sequence":"first","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6870-4595","authenticated-orcid":false,"given":"Md. Saiful","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"},{"name":"Department of Animal Science University of California\u2014Davis Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Ashek","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1400-1250","authenticated-orcid":false,"given":"Md. Liton","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhana Binte","family":"Ferdous","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al\u2010Muksit Mohammad Taufiquer","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medicine Rajshahi Medical College Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayedul","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5432-480X","authenticated-orcid":false,"given":"Md. Tanvir","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2018.12.017"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12562"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_2_11_5_1","first-page":"71","volume-title":"Pathogens and Toxins in Foods","author":"Beauchamp C. 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