{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:02:03Z","timestamp":1790326923643,"version":"4.1.0"},"reference-count":95,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rise of multidrug\u2010resistant (MDR) pathogens presents a major challenge to food safety, particularly in preservation approaches. Lactic acid bacteria (LAB) gain probiotic recognition by effectively controlling MDR pathogens using antimicrobial substances (AMSs) and bacteriocin\u2010like inhibitory substances (BLISs). The study evaluated the probiotic properties and AMS production potential of five LAB strains:\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    NBRC 3070 (LP),\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    ATCC 4356 (LA),\n                    <jats:italic>Lacticaseibacillus casei<\/jats:italic>\n                    ATCC 393 (LC),\n                    <jats:italic>Lacticaseibacillus rhamnosus<\/jats:italic>\n                    GG ATCC 53103 (LGG), and\n                    <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                    subsp.\n                    <jats:italic>lactis<\/jats:italic>\n                    ATCC 27673 (BAL). AMS was extracted from neutralized and concentrated cell\u2010free supernatant and tested for antimicrobial activities against common foodborne pathogens using minimum inhibitory concentrations (MICs). All LAB strains showed antagonistic activity, with LP demonstrating the most substantial antagonism. Four strains are resistant to multiple antibiotics but remain susceptible to azithromycin and tetracycline, while LP is resistant to all tested antibiotics. LP and LC demonstrated the highest probiotic potential based on cell surface hydrophobicity and aggregation abilities. Optimal AMS activity (85.36%\u201391.29%) occurred between 24 and 36\u2009h at 37\u00b0C or 28\u201348\u2009h at 30\u00b0C. LGG exhibited the highest AMS activity with the lowest MIC against\n                    <jats:italic>Salmonella enterica serovar<\/jats:italic>\n                    Typhimurium. Enzyme sensitivity confirmed the peptide nature of BLIS produced by BLIS from LP, LA, LC, and LGG. In conclusion, LAB strains demonstrated significant probiotic traits and the ability to produce AMS, especially BLIS, from four strains, with LP and LC showing the most potential. These findings support their application as natural biopreservation against MDR pathogens; however, further validation in the food system is required.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70052","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T08:10:56Z","timestamp":1790323856000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Probiotic Properties and Antimicrobial Substances Produced by Five Lactic Acid Bacteria Against Foodborne and Spoilage Pathogens"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9612-454X","authenticated-orcid":false,"given":"Md. Moklesur","family":"Rahman","sequence":"first","affiliation":[{"name":"Halal Products Research Institute Universiti Putra Malaysia UPM Serdang Selangor Malaysia"},{"name":"Poultry Research Center Bangladesh Livestock Research Institute (BLRI) Savar Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2461-271X","authenticated-orcid":false,"given":"Awis Qurni","family":"Sazili","sequence":"additional","affiliation":[{"name":"Halal Products Research Institute Universiti Putra Malaysia UPM Serdang Selangor Malaysia"},{"name":"Faculty of Agriculture Universiti Putra Malaysia UPM Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7625-3704","authenticated-orcid":false,"given":"Siti Aqlima","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Bimolecular Science Universiti Putra Malaysia UPM Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5321-4780","authenticated-orcid":false,"given":"Khalilah Abdul","family":"Khalil","sequence":"additional","affiliation":[{"name":"Faculty of Applied Sciences Universiti Teknologi MARA Shah Alam Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2280-5075","authenticated-orcid":false,"given":"Mohammad Rashedi","family":"Ismail\u2010Fitry","sequence":"additional","affiliation":[{"name":"Halal Products Research Institute Universiti Putra Malaysia UPM Serdang Selangor Malaysia"},{"name":"Faculty of Food Science and Technology Universiti Putra Malaysia UPM Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-5262","authenticated-orcid":false,"given":"Md. 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