{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T14:28:30Z","timestamp":1765376910816,"version":"3.46.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, we analyzed the BK polyomavirus (BKPyV) genome derived from three rodent cell lines established from experimentally induced tumors by injecting BKPyV into newborn rodents. Three cell lines (Vn\u2010324, In\u20101024, and Vn1919) were recently deposited in the JCRB Cell Bank (Japanese Collection of Research Bioresource Cell Bank). Vn\u2010324 was established from a hamster choroid plexus papilloma induced by BKPyV Gardner strain wild\u2010type 501 (\n                    <jats:italic>wt\u2010501<\/jats:italic>\n                    ). This cell line was reported to be negative for the large T\u2010antigen using indirect immunofluorescence. In this study, we examined the large T\u2010antigen expression using the reverse\u2010transcriptase\u2010polymerase chain reaction (RT\u2010PCR). In\u20101024 cells were established from hamster insulinoma. The strain of BKPyV from which were induced has not been reported. Vn1919 was established from a mouse ependymoma induced by the plaque morphology mutant 522 (\n                    <jats:italic>pm\u2010522<\/jats:italic>\n                    ). The noncoding control region (NCCR) of BKPyV derived from Vn\u2010324 genomic DNA and\n                    <jats:italic>wt<\/jats:italic>\n                    \u2010501 had the same structure, whereas the NCCR of BKPyV derived Vn1919 genomic DNA and\n                    <jats:italic>pm\u2010522<\/jats:italic>\n                    had the same structure. But the NCCR derived In\u20101024 was unique. We revealed that BKPyV derived from In\u20101024 genomic DNA had a large deletion in the viral proteins 1, 2, and 3 (VP1,(VP1, VP2, and VP3) coding region. This variant may be a proliferation\u2010defective mutant, which was expanded in human embryonic kidney cells with other mutants. These findings provide insights into the role of NCCR mutations in viral oncogenesis.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70061","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T03:40:23Z","timestamp":1757648423000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human Polyomavirus BK Genome Analysis in BKPyV Induced Rodent Cell Lines"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8463-4919","authenticated-orcid":false,"given":"Setsuko","family":"Shioda","sequence":"first","affiliation":[{"name":"National Institute of Biomedical Innovation, Health and Nutrition Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Kasai","sequence":"additional","affiliation":[{"name":"Cell Engineering Division, BioResource Research Center Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Midori","family":"Ozawa","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Innovation, Health and Nutrition Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azusa","family":"Ohtani","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Innovation, Health and Nutrition Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Iemura","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Innovation, Health and Nutrition Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Center for Stem Cell and Regenerative Medicine Tokyo Medical and Dental University (TMDU) Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arihiro","family":"Kohara","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Innovation, Health and Nutrition Ibaraki Osaka 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