{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:52:44Z","timestamp":1790970764312,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This systematic review and meta\u2010analysis compared bacterial semi\u2010quantification of respiratory samples from the BIOFIRE FILMARRAY Pneumonia (PN) Panels with quantitative and semi\u2010quantitative culture methods (qCMs). Fourteen studies comprising 1,654 samples were included. Across both bronchoalveolar lavage\u2010like and endotracheal aspirate\u2010like specimens, the BIOFIRE PN Panel reported consistently higher bacterial loads than qCMs, with pooled mean differences of 1.17 and 0.95 log, respectively. Discrepancies decreased as culture\u2010reported bacterial burden increased. The concordance rate in identifying the predominant pathogen was 94%, supporting the panel's clinical relevance. However, differential reporting at lower bacterial loads suggests that existing culture\u2010based thresholds may not translate directly to molecular diagnostics. These findings highlight the need for pathogen\u2010 and method\u2010specific interpretive thresholds to optimize the diagnostic utility of semi\u2010quantitative molecular results and inform antimicrobial stewardship decisions.<\/jats:p>","DOI":"10.1002\/mbo3.70086","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T06:00:38Z","timestamp":1767074438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Semi\u2010Quantitative Detection of Respiratory Pathogens: A Systematic Review and Meta\u2010Analysis of Results From the BIOFIRE FILMARRAY Pneumonia Panel and Culture"],"prefix":"10.1002","volume":"15","author":[{"given":"Benjamin","family":"Hommel","sequence":"first","affiliation":[{"name":"bioM\u00e9rieux, Marcy l'\u00c9toile  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oph\u00e9lie","family":"Hurtado","sequence":"additional","affiliation":[{"name":"bioM\u00e9rieux, Marcy l'\u00c9toile  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brooklyn","family":"Noble","sequence":"additional","affiliation":[{"name":"bioM\u00e9rieux Salt Lake City Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay","family":"Jones","sequence":"additional","affiliation":[{"name":"bioM\u00e9rieux Salt Lake City Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Allantaz","sequence":"additional","affiliation":[{"name":"bioM\u00e9rieux, Marcy l'\u00c9toile  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan T.","family":"Timbrook","sequence":"additional","affiliation":[{"name":"Barnes\u2010Jewish Hospital St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gennaro","family":"De Pascale","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze dell'Emergenza Anestesiologiche e della Rianimazione, Fondazione Policlinico Universitario A Gemelli IRCCS Rome Italy"},{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brunella","family":"Posteraro","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9780-7059","authenticated-orcid":false,"given":"Maurizio","family":"Sanguinetti","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze di Laboratorio ed Ematologiche Fondazione Policlinico Universitario A. Gemelli IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Albarillo F. S.Burdette S.Doron et al. Cap Clinical Pathway: Available at. 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