{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T21:10:09Z","timestamp":1769634609741,"version":"3.49.0"},"reference-count":88,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antibiotic resistance in\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    continues to rise, and its implications for healthcare delivery have intensified. We investigated the genetic basis of antimicrobial resistance (AMR), virulence genes, and associated plasmids in\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    isolates from a teaching hospital in Ghana. Antimicrobial susceptibility testing was performed using archived isolates. Whole genome sequencing was performed on a subset of the isolates, that were either multidrug resistant or extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2010producing. Bioinformatics analyses were performed for speciation, identification of AMR and virulence genes, and associated plasmids. The 100\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    isolates included in this study showed high phenotypic resistance to \u00df\u2010lactams and susceptibility to aminoglycosides. Nineteen of the 20 WGS isolates were genotypically identified using the housekeeping genes as\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (8\/20, 40%),\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (8\/20, 40%),\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    (2\/20, 10%), and\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    (1\/20, 5%). These strains harboured 139 unique antibiotic resistance genes (ARGs) encoding resistance against \u00df\u2010lactams (64\/139), aminoglycosides (23\/139), fluoroquinolones (45\/139), tetracyclines (37\/139), phenicols (27\/139), and sulphonamides (9\/139). Subsequent AST revealed that (74\/79, 94%) were ESBL producers, and (9\/79,11%) were carbapenem resistant\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    (CRE). The isolates expressed 8 categories of virulence factors (VFs), including effector delivery systems, adherence, and metabolic factors. Additionally, 26 unique plasmid replicons of both the I\u2010complex and colicin plasmids were detected. We observed phenotypic and genotypic evidence of antimicrobial resistance to common antibiotics in the isolates driven by\n                    <jats:italic>CTX\u2010M\u201015<\/jats:italic>\n                    and in some\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , the\n                    <jats:italic>NDM\u20101<\/jats:italic>\n                    . These findings highlight the urgent need to improve antibiotic stewardship, surveillance and control at the hospital.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70127","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T04:40:56Z","timestamp":1762749656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic and Genotypic Profile of\n                    <i>Enterobacteriaceae<\/i>\n                    Isolated at a Teaching Hospital in Ghana"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0764-0799","authenticated-orcid":false,"given":"Bismark","family":"Donkor","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences University of Cape Coast Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richael Odarkor","family":"Mills","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences University of Cape Coast Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philimon","family":"Mwintige","sequence":"additional","affiliation":[{"name":"Department of Microbiology Cape Coast Teaching Hospital Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberta Bedford","family":"Moses","sequence":"additional","affiliation":[{"name":"Laboratory Department Cape Coast Teaching Hospital Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail Asmah","family":"Brown","sequence":"additional","affiliation":[{"name":"Laboratory Department Cape Coast Teaching Hospital Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faustina","family":"Halm\u2010Lai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences University of Cape Coast Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oheneba Charles Kofi","family":"Hagan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, School of Medical Sciences, College of Health and Allied Sciences University of Cape Coast Cape Coast Central Region Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/336682a0"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-022-01090-2"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.glmedi.2024.100081"},{"issue":"1","key":"e_1_2_12_5_1","first-page":"517","article-title":"CARD 2020: Antibiotic Resistome Surveillance With the Comprehensive Antibiotic Resistance Database","volume":"48","author":"Alcock B. 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