{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:48:11Z","timestamp":1782470891950,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Phytopathogenic fungi such as\n                    <jats:italic>Fusarium solani<\/jats:italic>\n                    (\n                    <jats:italic>F. solani<\/jats:italic>\n                    ),\n                    <jats:italic>Rhizoctonia solani<\/jats:italic>\n                    (\n                    <jats:italic>R. solani<\/jats:italic>\n                    ), and\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    (\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    ) cause plant diseases, leading to significant yield losses. Lipopeptides (LPs), secondary metabolites produced by\n                    <jats:italic>Bacillus<\/jats:italic>\n                    strains, exhibit antifungal properties against these pathogens. In this study, the LP production potential of\n                    <jats:italic>Bacillus<\/jats:italic>\n                    isolates was investigated, and the best\u2010performing\n                    <jats:italic>Bacillus<\/jats:italic>\n                    sp. was selected for the optimization of the production medium and parameters. Hemolytic activities of\n                    <jats:italic>Bacillus<\/jats:italic>\n                    isolates were screened using blood agar, revealing that all 69 isolates exhibited hemolytic activity. The isolates were cultivated in Lysogeny Broth (LB) medium, and LP extractions were performed. The antifungal activity of LP extracts against\n                    <jats:italic>F. solani<\/jats:italic>\n                    ,\n                    <jats:italic>R. solani<\/jats:italic>\n                    , and\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    was assessed using the agar well diffusion method. The iturin and surfactin content of LP extracts was analyzed by quadrupole time\u2010of\u2010flight mass spectrometry. Five\n                    <jats:italic>Bacillus<\/jats:italic>\n                    sp. with high LP production potential were selected, and their cell growth was examined. Among them,\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    4\u2010Ka\u201022 demonstrated the highest cell growth. The cost\u2010effective optimized production medium was determined as 0.1% molasses, 1% soybean meal, 0.5% CaCl\n                    <jats:sub>2<\/jats:sub>\n                    , and 1% glycerol, with production parameters set at 30\u00b0C, 195\u2009rpm, pH 5, and a 4.3% inoculum ratio. Compared to LB medium, cell growth increased by 13.4\u2010fold, confirming successful optimization.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70130","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T12:26:05Z","timestamp":1778243165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Economical Bioprocess for Lipopeptide Production From\n                    <i>Bacillus subtilis<\/i>"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6540-639X","authenticated-orcid":false,"given":"Derya","family":"Maral\u2010G\u00fcl","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Graduate School of Natural and Applied Sciences Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3893-9876","authenticated-orcid":false,"given":"Z\u00fclal","family":"G\u00fcnay","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Graduate School of Natural and Applied Sciences Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baran","family":"Mis","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Faculty of Engineering Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0642-7676","authenticated-orcid":false,"given":"Rengin","family":"Eltem","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Faculty of Engineering Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12733"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3930112"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10532-018-9864-7"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.124827"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5772\/17184"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201600566"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/17518253.2021.1905080"},{"key":"e_1_2_10_9_1","first-page":"1","article-title":"The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains","volume":"8","author":"Dimki\u0107 I.","year":"2017","journal-title":"Frontiers in Microbiology"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-37618-8"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7705"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13484"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-812705-6.00006-X"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2022.101895"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-017-2530-2"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation10060323"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12061374"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2015.07.006"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2025.2499152"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26123488"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111171"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.17660\/ActaHortic.2005.669.9"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-015-0313-6"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.111143"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2018.10.012"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.4014\/kjmb.1207.07020"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092412-155650"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.4790"},{"key":"e_1_2_10_29_1","first-page":"51","article-title":"Characterization of Antibacterial Activity Produced by Bacillus spp. Isolated From Honey and Bee\u2010Associated Products Against Foodborne Pathogens","volume":"17","author":"Magdalena S.","year":"2020","journal-title":"Bioteknologi"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10123-024-00490-1"},{"key":"e_1_2_10_31_1","first-page":"91","article-title":"Lipopeptide Antibiotic Production by Bacillus velezensis KLP2016","volume":"8","author":"Meena K. 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