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While probiotics exhibit potential for developing such alternatives, the majority of antimicrobial studies focus on live cells or lysates and oral delivery. Notably, in dermatology, formulating products with live strains poses technical challenges due to stability issues in water\u2010based systems. Postbiotics, substances made from probiotics, offer a promising, stable, and safe alternative. This study addresses the gap by evaluating the antibacterial potential of cell\u2010free supernatants (CFSs) from six probiotic strains, with a specific focus on\n                      <jats:italic>Lacticaseibacillus paracasei<\/jats:italic>\n                      BGP1, against clinically relevant skin pathogens. CFSs were screened in vitro using agar well diffusion and broth microdilution assays against\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      ATCC 25923, methicillin\u2010resistant\n                      <jats:italic>S. aureus<\/jats:italic>\n                      (MRSA; UTMC 1442),\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      ATCC 27853, and\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      PAO1. GC\u2010MS analysis was used to identify bioactive compounds in the most promising CFS. Among the tested strains, BGP1 demonstrated both consistent inhibitory (MIC: 6.25\u2009mg\/mL) and bactericidal (MBC: 12.5\u2009mg\/mL) effects. GC\u2010MS analysis identified palmitic acid (33.24%) and stearic acid (46.45%) as dominant bioactive compounds. These findings provide novel evidence that postbiotic metabolites from\n                      <jats:italic>L. paracasei<\/jats:italic>\n                      BGP1 represent a promising, broad\u2010spectrum, stable, and nonliving candidate to conventional therapies for antibiotic\u2010resistant skin wound infections. Further in vivo research is needed to evaluate their therapeutic potential and formulation in clinical settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mbo3.70137","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T09:38:16Z","timestamp":1765186696000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Broad\u2010Spectrum Antibacterial Activity of Postbiotic From\n                    <i>Lacticaseibacillus paracasei<\/i>\n                    BGP1 Against Multidrug\u2010Resistant Skin Wound Pathogens"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9442-1145","authenticated-orcid":false,"given":"Farideh","family":"Mohammadhosseinzadeh","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Biology, College of Science University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0758-4710","authenticated-orcid":false,"given":"Ehsan","family":"Arefian","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Biology, College of Science University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4537-2601","authenticated-orcid":false,"given":"Mouj","family":"Khaleghi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Shahid Bahonar University of Kerman Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoda","family":"Keshmiri Neghab","sequence":"additional","affiliation":[{"name":"Department of Medical Laser, Medical Laser Research Center Yara Institute, ACECR Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasim","family":"Kashef","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Biology, College of Science University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2018.03.009"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10121574"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-020-09631-0"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-020-09673-4"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-024-01363-5"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MRM.0000000000000067"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.02037"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S232982"},{"key":"e_1_2_12_10_1","first-page":"149","article-title":"\u2018Antibiotic Susceptibility Testing by a Standardized Single Disc Method","volume":"45","author":"Bauer A. 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