{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:16:35Z","timestamp":1791980195370,"version":"4.4.0"},"reference-count":71,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The emergence and dissemination of aquatic pathogens pose significant risks to farmed species.\n                    <jats:italic>Francisella halioticida<\/jats:italic>\n                    , initially reported in abalones and Yesso scallops, was recently isolated from mussels in France, with some isolates showing high virulence. This study aimed to characterize and compare several\n                    <jats:italic>F. halioticida<\/jats:italic>\n                    isolates from mussels using phenotypic and genotypic approaches. Phenotypic analysis was performed using growth curves, biochemical profiles (API strips), and morphology assessed by electron microscopy. Genetic analysis has been performed through whole\u2010genome comparison using classification methods and virulence markers seeking. Phenotypic analyses highlighted similarities among FR22 isolates and notable differences with FR21 and AG1. Notably, AG1 displayed distinct features. Antibiotic resistance profiling revealed the species' capacity to withstand multiple antimicrobial agents with various modes of action. Complete, circular genomes were assembled and compared using targeted and untargeted approaches. These analyses confirmed the affiliation of FR22 isolates with the\n                    <jats:italic>F. halioticida<\/jats:italic>\n                    species, while FR21 and AG1 taxonomy need to be further investigated. Virulence factor screening revealed the presence of secretion system components (types I, IV, and VI) in all isolates. A novel variant of the\n                    <jats:italic>Francisella<\/jats:italic>\n                    Pathogenicity Island (FPI) was described, shared by all virulent isolates. However, this FPI was absent in the low virulence isolate FR22b. In conclusion, this study discriminates against\n                    <jats:italic>F. halioticida<\/jats:italic>\n                    isolates and proposes new hypotheses on their virulence, contributing to improved detection tools and expanding our understanding of this emerging aquatic pathogen.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70172","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T10:31:16Z","timestamp":1764325876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phenotypical and Genomic Characterization of the Mollusk Pathogen\n                    <i>Francisella halioticida<\/i>"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3622-1468","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Bouras","sequence":"first","affiliation":[{"name":"Marine Ecosystems and Organisms Research Laboratory (MERSEA), Esplanade de la Paix University of Caen Normandy Caen France"},{"name":"Research Department LAB\u00c9O Caen Cedex 4 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Quesnelle","sequence":"additional","affiliation":[{"name":"Research Department LAB\u00c9O Caen Cedex 4 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7273-6215","authenticated-orcid":false,"given":"Suzanne","family":"Trancart","sequence":"additional","affiliation":[{"name":"Research Department LAB\u00c9O Caen Cedex 4 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Goux","sequence":"additional","affiliation":[{"name":"CMAbio3 (Centre de Microscopie Appliqu\u00e9e \u00e0 la Biologie), SF EMerode, Esplanade de la Paix UNICAEN, Normandie Universit\u00e9 Caen France"},{"name":"CNRS, UMR 6508 CRISMAT, ENSICAEN UNICAEN, Normandie Universit\u00e9 Caen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Louis","family":"Blin","sequence":"additional","affiliation":[{"name":"Synergie Mer et Littoral (SMEL) Zone Conchylicole Blainville\u2010sur\u2010mer France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Savary","sequence":"additional","affiliation":[{"name":"Comit\u00e9 R\u00e9gional de Conchyliculture (CRC) Normandie\u2010Mer du Nord Gouville\u2010sur\u2010Mer France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryline","family":"Houssin","sequence":"additional","affiliation":[{"name":"Marine Ecosystems and Organisms Research Laboratory (MERSEA), Esplanade de la Paix University of Caen Normandy Caen France"},{"name":"Research Department LAB\u00c9O Caen Cedex 4 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Zatylny\u2010Gaudin","sequence":"additional","affiliation":[{"name":"Marine Ecosystems and 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