{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T20:43:07Z","timestamp":1769632987171,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Emerging evidence suggests that\n                    <jats:italic>OBSCN<\/jats:italic>\n                    , a giant cytoskeletal protein gene, plays multifaceted roles in cancer progression, yet its impact on gastric cancer (GC) remains poorly understood. Through integrative analysis of multi\u2010omics datasets, we observe a close relationship between\n                    <jats:italic>OBSCN<\/jats:italic>\n                    expression and outcome of immunotherapy. Besides, elevated expression of\n                    <jats:italic>OBSCN<\/jats:italic>\n                    strongly associated with adverse disease free survival (DFS). Tumor\u2010resident microbes, such as\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    , can impact the expression of microRNAs (miRNAs) targeting\n                    <jats:italic>OBSCN<\/jats:italic>\n                    . In terms of genomic alterations, mutational status of\n                    <jats:italic>OBSCN<\/jats:italic>\n                    is substantially associated with the alpha\u2010 and beta\u2010diversity of intratumoral microbiome and patients with mutated\n                    <jats:italic>OBSCN<\/jats:italic>\n                    exhibit elevated higher tumor mutational burden (TMB) and better response to immunotherapy. Furthermore, machine learning models based on the\n                    <jats:italic>OBSCN<\/jats:italic>\n                    mutation\u2010related gene signatures (OMRGS) achieve outstanding performance in prediction of response to immune checkpoint inhibitors. In summary, our findings position\n                    <jats:italic>OBSCN<\/jats:italic>\n                    as a novel molecular nexus linking genomic alterations, intratumoral microbiome dysbiosis, and immune infiltration in GC, providing a rationale for future biomarker\u2010driven therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70186","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T03:30:51Z","timestamp":1764559851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Omics Analysis Reveals\n                    <i>OBSCN<\/i>\n                    as a Key Modulator of Tumor Microenvironment, Microbial Signatures and Clinical Outcomes in Gastric Cancer"],"prefix":"10.1002","volume":"14","author":[{"given":"Hongfang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pathology Yidu Central Hospital of Weifang Weifang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Physical Examination Center, Daqing Longnan Hospital, The Fifth Affiliated Hospital of Qiqihar Medical College Daqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Chifeng Municiple Hospital Chifeng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Emergency Shanghai Tenth People's Hospital, School of Medicine Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Emergency, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 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