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Biofilms, structured microbial communities embedded in a protective extracellular matrix, underlie many chronic and recurrent infections, including endocarditis, urinary tract infections, cystic fibrosis lung disease, and device\u2010related infections. Conventional antibiotics often fail in these contexts, and the discovery pipeline for novel agents remains limited. Nanotechnology has therefore emerged as a promising alternative, offering unique physicochemical features that enable enhanced penetration into biofilm matrices, improved drug stability, and targeted delivery of therapeutic agents. Diverse nanosystems, including metallic, polymeric, lipid\u2010based, and ligand\u2010functionalized platforms, have shown encouraging results in vitro and in vivo, demonstrating superior biofilm disruption and bacterial eradication compared with conventional therapies. Nevertheless, translating these advances into clinical practice remains challenging. 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