{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:54:45Z","timestamp":1773014085414,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008660","name":"Kurozumi Medical Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008660","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21fk0108133"],"award-info":[{"award-number":["JP21fk0108133"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the usefulness of the IR Biotyper, which types bacterial strains using Fourier transform infrared (FT\u2010IR) spectroscopy, against extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2010 and metallo\u2010\u03b2\u2010lactamase (MBL)\u2010producing Enterobacterales. Sixty\u2010six clinical isolates (20 ESBL\u2010producing\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    , 15 IMP\u2010producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , and 31 IMP\u2010producing\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    complex isolates) were analyzed using the IR Biotyper, pulsed\u2010field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and whole\u2010genome single\u2010nucleotide polymorphism (wgSNP) analyses and the results were compared. Of the 20 ESBL\u2010producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strains analyzed, the IR Biotyper detected three clusters. Of these clusters, two were determined as respective clusters by PFGE and wgSNP analyses, and the strains included in each cluster showed the same STs. The IR Biotyper detected three clusters in the analysis of 15 IMP\u2010producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strains. Of these clusters, strains in the two clusters showed high concordance with PFGE, MLST, and wgSNP analyses. The IR Biotyper identified six clusters among the IMP\u2010producing\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    complex isolates. These results were fully concordant with those of PFGE, MLST, and wgSNP analyses in the two clusters. The range of adjusted Rand index was 0.734\u20130.967 between the IR Biotyper and PFGE and 0.658\u20130.857 between the IR Biotyper and MLST or wgSNP analyses. This study demonstrated the performance of IR Biotyper for the detection of clonal similarities in ESBL\u2010 and IMP\u2010producing Enterobacterales and it might be useful for outbreak investigation.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70218","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:39:45Z","timestamp":1770053985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Typing of Extended\u2010Spectrum \u03b2\u2010Lactamase (ESBL)\u2010 and Metallo\u2010\u03b2\u2010Lactamase (MBL)\u2010Producing Enterobacterales Using Fourier Transform Infrared (FT\u2010IR) Spectroscopy"],"prefix":"10.1002","volume":"15","author":[{"given":"Yasuhide","family":"Kawamoto","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Hospital  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2296-6539","authenticated-orcid":false,"given":"Kosuke","family":"Kosai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Hospital  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Ota","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Hospital  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4825-5657","authenticated-orcid":false,"given":"Fujiko","family":"Mitsumoto\u2010Kaseida","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norihito","family":"Kaku","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Hospital  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-5692","authenticated-orcid":false,"given":"Hiroo","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Hospital  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Izumikawa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Mukae","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsunori","family":"Yanagihara","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nagasaki University Hospital  Nagasaki Nagasaki Japan"},{"name":"Department of Laboratory Medicine Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2019.282"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4178-4182.2005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2014.11.012"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.10.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000577"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13709"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1068872"},{"key":"e_1_2_11_9_1","unstructured":"Japanese Association of Medical Technologists.2003. 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