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There is currently no commercially available vaccine, and prolonged antibiotic treatment may increase drug resistance. We developed a\n                    <jats:italic>Treponema pallidum<\/jats:italic>\n                    membrane protein C (TMPC)\u2010based multi\u2010epitope vaccine targeting nine TMPC\u2010positive streptococcal species dominated by\n                    <jats:italic>S. anginosus<\/jats:italic>\n                    . B\u2010cell and T\u2010cell epitopes were chosen based on their binding affinity, antigenicity, immunogenicity, and safety, with adjuvants and linker sequences improving construct stability and immune response. Immune simulations predicted robust humoral and cellular responses, such as cytokine production and memory cell activation. Molecular docking and molecular dynamics analysis further confirmed stable interactions between the vaccine construct and key immune receptors (HLA\u2010A*02:01, HLA\u2010DRB1*01:01, TLR2, and TLR4). The antigen was further modified as a messenger RNA vaccine to enhance cytotoxic T\u2010cell induction; however, animal research is needed to confirm its immunogenicity and protective effectiveness.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70230","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T04:33:08Z","timestamp":1770784388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Computational Validation of Multi\u2010Epitope mRNA Vaccine Targeting\n                    <i>Streptococcus anginosus<\/i>\n                    Surface Protein (TMPC) as an Effective Alternative Treatment to Reduce Gastric Cancer"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9201-8069","authenticated-orcid":false,"given":"Fei","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, National Key Clinical Specialty, 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