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In this study, zinc oxide\u2010magnesium oxide nanocomposite (ZnO\u2010MgO NCs) were biologically created employing the\n                    <jats:italic>Sarocladium kiliense<\/jats:italic>\n                    PV248633.1 fungal strain. Ultraviolet\u2010visible spectroscopy confirmed nanoparticle formation through characteristic absorption peaks, while Fourier\u2010transform infrared spectroscopy identified functional groups associated with metal\u2010oxygen bonds. Also, the nanoparticle size and surface characteristics Transmission electron microscopy and dynamic light scattering analyzes revealed an average particle size of 35\u2009nm. Biological assessments demonstrate potent antitumor activity (IC\u2085\u2080\u2009=\u200978.1\u2009\u00b5g\/mL) against MCF\u20107 breast cancer cells, alongside minimal cytotoxicity against normal WI\u201038 cells (IC\u2085\u2080\u2009=\u2009218.7\u2009\u00b5g\/mL), indicating selective toxicity. Antibacterial evaluations showed particularly high efficacy against\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    occasionally surpassing the performance of cefepime. Dose\u2010dependent antimicrobial activity was confirmed through well diffusion and broth microdilution techniques, with minimum inhibitory concentrations highlighting strong bactericidal potential. Biofilm inhibition assays revealed strain\u2010specific responses, with\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    being the most susceptible. Furthermore, the synergistic combination of the biosynthesized nanocomposite with cefepime enhanced antibacterial outcomes against selected isolates, suggesting potential for addressing drug resistance. Overall, the mycogenic ZnO\u2010MgO NCs synthesized from\n                    <jats:italic>S. kiliense<\/jats:italic>\n                    exhibited substantial anticancer, antibacterial, and antibiofilm properties, locating this nanocomposite as a promising candidate for biomedical purposes.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70235","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T13:40:51Z","timestamp":1771508451000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Green and Ecofriendly Mycosynthesis of Nanocomposite Based on Zinc and Magnesium Oxide Nanoparticles Using Endophytic\n                    <i>Sarocladium kiliense<\/i>\n                    : Characterization, Anticancer Activity, Antimicrobial and Antibiofilm Activities"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4025-8586","authenticated-orcid":false,"given":"Samy","family":"Selim","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences Jouf University Sakaka Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathy M.","family":"Elkady","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Department, Faculty of Pharmacy (Boys) Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-7912","authenticated-orcid":false,"given":"Ebrahim","family":"Saied","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, Faculty of Science Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0024-3606","authenticated-orcid":false,"given":"Amr H.","family":"Hashem","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, Faculty of Science Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2410-5168","authenticated-orcid":false,"given":"Amer M.","family":"Abdelaziz","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, Faculty of Science Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"Abdulrahman","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Department, Faculty of Pharmacy (Boys) Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7267-9636","authenticated-orcid":false,"given":"Faisal","family":"Alsenani","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy Umm Al\u2010Qura University Makkah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2848-2932","authenticated-orcid":false,"given":"Omar Awad","family":"Alsaidan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy Jouf University Sakaka Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4007-4023","authenticated-orcid":false,"given":"Sami I.","family":"Alzarea","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Jouf University Sakaka Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0945-9224","authenticated-orcid":false,"given":"Yousef Alhaj","family":"Hamoud","sequence":"additional","affiliation":[{"name":"College of Hydrology and Water Resources Hohai University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiba","family":"Shaghaleh","sequence":"additional","affiliation":[{"name":"School of Energy and Environment Southeast University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6693-4530","authenticated-orcid":false,"given":"Mohammed","family":"Aufy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Division of Pharmacology and Toxicology University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"1","article-title":"Cowpea Leaf and Pod Spots Caused by Periconia igniaria and Their Potential Control Using Biosynthesized Zinc Oxide and Magnesium Oxide Nanoparticles","volume":"12","author":"Abdel\u2010Samad F. 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