{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T13:12:30Z","timestamp":1777036350865,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nocardiosis is an infectious disease caused by several\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species, among which\n                    <jats:italic>Nocardia cyriacigeorgica<\/jats:italic>\n                    is one of the most frequently isolated species in the clinic. Albeit most isolates of this species are susceptible to standard treatment combining trimethoprim and sulfamethoxazole, resistance has been reported, necessitating alternative or combination therapies. \u03b2\u2010lactam antibiotics are of particular interest in this context. In this study, we aimed to address the \u03b2\u2010lactam susceptibility profile of a clinical strain of\n                    <jats:italic>N. cyriacigeorgica<\/jats:italic>\n                    and assessed whether it correlated with the enzymatic activity of purified \u03b2\u2010lactamase of the strain. We herein established that the strain is highly susceptible to imipenem and ceftriaxone, moderately susceptible to meropenem and resistant to amoxicillin. The resistance could be counteracted by \u03b2\u2010lactamase inhibitors from two distinct chemical classes: vaborbactam, and avibactam while clavulanate was less potent. We demonstrated that the \u03b2\u2010lactam susceptibility of the strain is in direct line with the enzymatic activity of purified NCY\u20101, a class A \u03b2\u2010lactamase. NCY\u20101 was indeed only active with amoxicillin but displayed poor activity towards other classes of \u03b2\u2010lactams. The NCY\u20101 activity could be inhibited\n                    <jats:italic>in vitro<\/jats:italic>\n                    by vaborbactam, clavulanate, and avibactam. We consolidated these data by determining the high\u2010resolution structure of NCY\u20101 bound to avibactam. The structural analysis supported a conserved inhibitor binding site among other\n                    <jats:italic>Nocardia<\/jats:italic>\n                    class A \u03b2\u2010lactamases strongly suggesting a broad inhibition spectrum of avibactam across\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70267","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T10:47:11Z","timestamp":1774003631000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["NCY\u20101 \u03b2\u2010Lactamase Activity Correlates With Antimicrobial Susceptibility of a Clinical Strain of\n                    <i>Nocardia cyriacigeorgica<\/i>"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1880-8175","authenticated-orcid":false,"given":"Julie","family":"Couston","sequence":"first","affiliation":[{"name":"IRIM, Univ Montpellier, CNRS Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1753-7229","authenticated-orcid":false,"given":"Ath\u00e9na\u00efs","family":"Le Marchand","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale Multisites, Service des Bact\u00e9riologie, Laboratoire de Biologie M\u00e9dicale de R\u00e9f\u00e9rence des Nocardioses, Institut des Agents Infectieux Hospices Civils de Lyon, H\u00f4pital de la Croix\u2010Rousse, Centre de Biologie Nord Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5144-1987","authenticated-orcid":false,"given":"Elisabeth","family":"Hodille","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale Multisites, Service des Bact\u00e9riologie, Laboratoire de Biologie M\u00e9dicale de R\u00e9f\u00e9rence des Nocardioses, Institut des Agents Infectieux Hospices Civils de Lyon, H\u00f4pital de la Croix\u2010Rousse, Centre de Biologie Nord Lyon France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), INSERM U1111, CNRS UMR5308, ENS Lyon Universit\u00e9 Lyon 1 Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6846-4053","authenticated-orcid":false,"given":"Charlotte","family":"Genestet","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale Multisites, Service des Bact\u00e9riologie, Laboratoire de Biologie M\u00e9dicale de R\u00e9f\u00e9rence des Nocardioses, Institut des Agents Infectieux Hospices Civils de Lyon, H\u00f4pital de la Croix\u2010Rousse, Centre de Biologie Nord Lyon France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), INSERM U1111, CNRS UMR5308, ENS Lyon Universit\u00e9 Lyon 1 Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4753-0677","authenticated-orcid":false,"given":"Brune","family":"Joannard","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale Multisites, Service des Bact\u00e9riologie, Laboratoire de Biologie M\u00e9dicale de R\u00e9f\u00e9rence des Nocardioses, Institut des Agents Infectieux Hospices Civils de Lyon, H\u00f4pital de la Croix\u2010Rousse, Centre de Biologie Nord Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5362-6099","authenticated-orcid":false,"given":"J\u00e9r\u00f4me","family":"Feuillard","sequence":"additional","affiliation":[{"name":"IRIM, Univ Montpellier, CNRS Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Benito","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale Multisites, Service des Bact\u00e9riologie, Laboratoire de Biologie M\u00e9dicale de R\u00e9f\u00e9rence des Nocardioses, Institut des Agents Infectieux Hospices Civils de Lyon, H\u00f4pital de la Croix\u2010Rousse, Centre de Biologie Nord Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8193-1437","authenticated-orcid":false,"given":"Oana","family":"Dumitrescu","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale Multisites, Service des 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