{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:47:28Z","timestamp":1782470848183,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    IMP\u20101700 was developed as a compound designed to target bacterial DNA repair\u2010associated pathways, including homologous recombination mediated by the AddAB\/RecBCD system. While its antibacterial properties are well established, the potential effects on mammalian noncancerous and tumor cells under genotoxic stress remain to be elucidated. This study investigates the impact of IMP\u20101700 and its synergistic effect in combination with ciprofloxacin (CPX) and X\u2010ray radiation in bacterial cultures and mammalian cell lines. In\n                    <jats:italic>Staphylococcus aureus (S. aureus)<\/jats:italic>\n                    , neither IMP\u20101700 (5\u2009\u00b5M) nor CPX (15\u2009\u00b5M) as monotherapy significantly reduced bacterial growth; their combination produced a substantial reduction in bacterial counts, and IMP\u20101700 further enhanced killing when combined with 10\u2009Gy X\u2010ray irradiation, resulting in a 77% decrease, demonstrating synergistic antibacterial activity. In mammalian cells, X\u2010ray cytotoxicity was cell\u2010type dependent: noncancerous NIH\u20103T3 fibroblasts showed no significant effect, while B16.F10 melanoma cells displayed delayed sensitivity, MDA\u2010MB\u2010231 breast cancer cells an intermediate response, and HepG2 hepatocellular carcinoma cells marked radioresistance. IMP\u20101700 enhanced radiation\u2010induced cytotoxicity across all cell lines, particularly at 72\u2009h post\u2010irradiation. These findings suggest that although IMP\u20101700 was designed to target bacterial DNA repair pathways, it can enhance sensitivity to genotoxic stress in both bacterial and mammalian systems. Further mechanistic investigation and safety evaluation are required before potential therapeutic application.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70270","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T12:27:42Z","timestamp":1777292862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Effects of IMP\u20101700, Ciprofloxacin, and X\u2010Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial\u2010Resistant Bacteria"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4949-8965","authenticated-orcid":false,"given":"Ida Vang","family":"Andersen","sequence":"first","affiliation":[{"name":"Department of Clinical Physiology and Nuclear Medicine &amp; Cluster for Molecular Imaging, Copenhagen University Hospital \u2013 Rigshospitalet &amp; Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ane Beth","family":"Sloth","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology and Nuclear Medicine &amp; Cluster for Molecular Imaging, Copenhagen University Hospital \u2013 Rigshospitalet &amp; Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie Caroline Skuladottir","family":"B\u00f8gestad","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology and Nuclear Medicine &amp; Cluster for Molecular Imaging, Copenhagen University Hospital \u2013 Rigshospitalet &amp; Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian Bernard Matthijs","family":"Poulie","sequence":"additional","affiliation":[{"name":"Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Skovsbo","family":"Clausen","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology and Nuclear Medicine &amp; Cluster for Molecular Imaging, Copenhagen University Hospital \u2013 Rigshospitalet &amp; Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1012-8644","authenticated-orcid":false,"given":"Umberto Maria","family":"Battisti","sequence":"additional","affiliation":[{"name":"Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias M.","family":"Herth","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology and Nuclear 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