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This study characterized the antibiotic resistance (AR) and virulence of\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates from chicken and beef (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009109) imported for retail sale in Kasane, Botswana. In this relatively pristine environment, commercial beef and chicken production is absent, resulting in reliance on imports, creating concerns that multidrug\u2010resistant (MDR) and pathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    may be introduced through the food supply sourced from distant regions.\n                    <jats:italic>E. coli<\/jats:italic>\n                    was isolated from 54.7% of samples (63\/109). Antibiotic susceptibility testing against a panel of 12 antibiotics revealed resistance to 11 antibiotics, with multiple combinations of resistance phenotypes identified. Higher levels of MDR were found in chicken isolates (45.5%) compared to beef (13.3%), with the highest resistance rates observed for tetracycline, trimethoprim\/sulfamethoxazole, and doxycycline. Genomic analysis of eight MDR isolates revealed diverse sequence types, including diarrheagenic and extraintestinal\u2010associated serotypes. The latter has critical implications in health systems where this clinical presentation may not be investigated with foodborne pathogen exposures. Plasmid\u2010borne AR genes with conjugation\u2010associated genes were detected in most isolates, suggesting that some AR genes may be horizontally transferable by plasmid conjugation. Several isolates clustered with human and chicken isolates from around the globe, highlighting the high potential for retail beef and chicken products to harbor MDR pathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    , including emerging pathogens, and to introduce those microbes and associated AR attributes into new ecosystems.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70273","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T11:21:28Z","timestamp":1774869688000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Imported Retail Beef and Chicken Meat Products Serve as Reservoirs for Emerging Antibiotic\u2010Resistant Pathotypes of\n                    <i>Escherichia coli<\/i>\n                    in Pristine Areas Free From Agricultural Activity"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1434-6577","authenticated-orcid":false,"given":"Saehah","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology Virginia Tech Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen A.","family":"Alexander","sequence":"additional","affiliation":[{"name":"CARACAL\/Chobe Research Institute Kasane Botswana"},{"name":"Department of Fisheries and Wildlife Virginia Tech Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auja","family":"Bywater","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology Virginia Tech Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galaletsang","family":"Dintwe","sequence":"additional","affiliation":[{"name":"CARACAL\/Chobe Research Institute Kasane Botswana"},{"name":"Department of Fisheries and Wildlife Virginia Tech Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashton N.","family":"Sies","sequence":"additional","affiliation":[{"name":"Department of Biology University of Regina Regina SK Canada"},{"name":"Institute for Microbial Systems and Society, Faculty of Science University of Regina Regina SK Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"Haidl","sequence":"additional","affiliation":[{"name":"Department of Biology University of Regina Regina SK Canada"},{"name":"Institute for Microbial Systems and Society, Faculty of Science University of Regina Regina SK Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D. 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