{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T15:36:45Z","timestamp":1783784205361,"version":"3.55.0"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    endophthalmitis is a rapidly progressing intraocular infection that often results in poor visual outcomes due to extensive retinal damage. Microglia are resident innate immune cells in the brain and retina that play critical roles in neurological and ocular diseases. To investigate the functional role and mechanisms of microglia during\n                    <jats:italic>B. cereus<\/jats:italic>\n                    infection, we established an in vitro microglial infection model using murine BV2 and human HMC3 cells.\n                    <jats:italic>B. cereus<\/jats:italic>\n                    infection reduced microglial viability and induced membrane rupture. Transcriptome analysis revealed enrichment of inflammatory and cell death. Flow cytometric screening identified that the RIPK1 inhibitor Nec\u20101 rescued cell death. Mechanistically,\n                    <jats:italic>B. cereus<\/jats:italic>\n                    increased phosphorylation of RIPK3 and MLKL, which was abolished by Nec\u20101. Moreover, Nec\u20101 suppressed\n                    <jats:italic>B. cereus<\/jats:italic>\n                    \u2010induced secretion of IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1. In summary, this study demonstrates that\n                    <jats:italic>B. cereus<\/jats:italic>\n                    induces microglial necroptosis by activating the RIPK3\/MLKL pathway, providing a new mechanism for neuroinflammation caused by related infections.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70276","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T23:31:52Z","timestamp":1774567912000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Bacillus cereus<\/i>\n                    Induces Necroptosis in Microglia via the RIPK1\/3\u2010MLKL Pathway"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9239-504X","authenticated-orcid":false,"given":"Jing","family":"Yang","sequence":"first","affiliation":[{"name":"National Clinical Research Center for Ocular Diseases, EyeHospital Wenzhou Medical University Wenzhou 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